جستجوی مقالات مرتبط با کلیدواژه « hairy root » در نشریات گروه « زیست شناسی »

Hairy root induction and production in Dracocephalum kotschyi Boiss. was investigated as an environmental and tissue culture technique for producing secondary metabolites. For this purpose, an in vitro experiment was carried out in the form of a completely randomized design (CRD) with four replicates. The best strain of Agrobacterium rhizogenes among MSU, A7 and A4-N strains from four-week-old leaves of D. kotschyi and the best age of leaves for inoculation with A. rhizogenes from two, three and four-week-old seedlings were chosen. In order to induce hairy roots, the most appropriate culture medium in the co-cultivation and post-co-cultivation phases was selected among the MS and MS culture media without macro elements (NH4NO3, KNO3, CaCl2, and KH2PO4) and the MS and MS culture media containing L-glutamine (0.1 g/L), respectively. The highest percent transgenic hairy root was observed in the MSU strain (90%), four-week old leaf explants of D. kotschyi seedlings (82.28%) and MS medium without macro elements in the co-cultivation stage, and MS medium containing L-glutamine in the post-co-cultivation stage (75.78%). PCR analysis also verified the transgenic nature of the hairy roots using rolC, aux1 and virD specific primers. The results showed that hairy root production in D. kotschyi depended on the type of A. rhizogenes strain, the age of the explant, and the MS medium. Nevertheless, the length of the produced hairy root in D. kotschyi did not depend on the type of A. rhizogenes strain and the age of the explant. The results of this study can be used in the production of medicinal compounds from this plant, such as rosmarie acid, through the optimized hairy root technique.

Astragalus compactus L. seeds were collected fromTehran of Iran. Plants were grown in growth chamber under 16 h daily light periods and leaves, stems, prdicle and cotyledon leaves explantn from three- week- old sterilized seedlings cut and infected with Agrobacterium rhizogenes Strains 15834, 2659 and WT.Hairy roots obtained from leave and stem explants but did not induce hairy roots of cotyledon and pedicle explants of Astragalus compactus.Strain 2659 resulted in the highest amount of hairy root formation in this Species. Strain 15834 was beneficial but not as effective as strain 2659. In contrast to these two strain, strain WT did not induce hairy roots in any of the explants used in this study. Thus, this strain seems to be totally avirulent to Astragalus compactus.