جستجوی مقالات مرتبط با کلیدواژه « نشانگر آگاهی بخش » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

The olive is considered as one of the most important horticultural crops, with more abundant number of cultivars. Thus, the aim of this research was to study the genetic diversity in a set of olive cultivars in Taroum station of Tran. In this research, 25 CBDP markers were used to study the genetic diversity and relationships of 20 promising and commercial Iranian and foreign varieties of olive. The 25 used primers amplified a total of 755 polymorph alleles, and the mean of PIC and MI indices were 0.941 and 5.64, respectively, indicating the high efficiency and differentiation power of CBDP markers. The genetic parameters of Nei and Shannon indices were higher in foreign cultivars than those of Iranian cultivars. The high gene flow (4.46) was observed among olive cultivars, which confirmed the low values of the inter-population differentiation (Gst) and FsT indices. Analysis of molecular variance (AMOVA) differentiated the total genetic variation into inter-group (Iranian and foreign cultivars) (17%) and within-group (83%) diversity. The cluster analysis using Dice coefficient and UPGMA method divided the Iranian and foreign cultivars into five groups, which the promising cultivar T24 and Koroneiki with the maximum difference with others were placed in separate groups. The Principal Coordinate Analysis (PCoA) was able to distinguish promising Iranian cultivars from the other commercial cultivars. The population structure analysis confirmed the results of the cluster and PCoA analysis and clearly separated the two groups of Iranian and foreign cultivars. In conclusion, it was observed that the promising cultivar T24 with having a genetic similarity to both groups of Iranian and foreign cultivars can be a suitable candidate for commercialization with complementary characteristics of both groups. Also, it is recommended to use this marker to study the genetic diversity of other olive varieties.

Physiological activity and biochemical changes continues in postharvest of cut flowers. These conditions reduce the longevity of flowers and accelerate the senescence process. In order to identify informative AFLP markers for description of senescence in Chrysanthemum, association analysis was used. The relationships between biochemical traits and molecular markers based on 25 primer combinations (EcoRI and MseI) and genetic structure of 44 chrysanthemum genotypes were evaluated. The results of correlation coefficient indicated a positive and significant correlation between protein concentration and lipid peroxidation (r=0.304**) as indicators of senescence. Results of MLM association analysis model for 5 variable showed that 143 AFLP markers were found to be associated with biochemical traits in Chrysanthemum, whereas GLM model identified 419 markers for biochemical traits. Moreover, we were able to identify 49 markers associated with malondialdehyde. However, only 14 AFLP markers associated with petal senescence. The strongest association was detected between AFLP markers of M-CAC/E-AGA13, with superoxide dismutase enzyme which explained 47 percent of variation. The most variation of petal senescence (32%) was accounted by M-CTT/E-AAC35 marker. Also, our results showed that some of the markers were co-associated with different chemical traits. Moreover, informative markers such as M-CTG/E-ACC16, M-CAA/E-AAG40 and M-CTG/E-AGA3 that have been shown to significant correlation with several traits can used for breeding programs and other analyses associated with future studies of Chrysanthemum if they contribute to increase longevity in other experiments.