جستجوی مقالات مرتبط با کلیدواژه « فیلوژنی » در نشریات گروه « شیلات »

The genus Peronia Fleming, 1822 by having 14 species, is one of the most diverse genera of the Onchidiidae family, which is highly distributed in the waters of the Persian Gulf and the Sea of ​​Oman. Despite their important ecological role in these areas, unfortunately, they have not been well studied so far. In this study, while describing the morphological characteristics of two species of this genus, Peronia madagascariensis (Labbé, 1934) and P. verruculata (Cuvier, 1830) with the help of a fragment sequence of a cytochrome oxidase gene, their status was determined. In this study, for the first time radulla belonging to the members of this genus in the northwestern Indian Ocean region were photographed and P. madagascariensis was reported from Iranian waters of the Oman Sea.

Some species of phytoplankton under unfavorable environmental conditions and in their sexual life cycle produce resting cyst that settle to the bottom sediments and become motile in the water column when suitable conditions provided. Phytoplankton species were studied in two stages of motile and cyst for accurate identification of morphology, because some similar cysts may convert to a different phytoplankton and vice versa. The objectives of this study were to culture cysts that isolated from sediments of Lipar zone located in the southeast coast of Iran and to identify excysted samples. Sediment samples from Lipar zone were collected by Ekman grab with 225 cm2 area in 2015. Unknown live and orbicular single cysts were isolated from sediments and cultured in sterile conditions in phycolab using f2 medium under 12 hours light and 12 hours dark cycles at 25±1°C. Assessment of excysted plankton morphology revealed that the cyst is belonged to Dunaliella salina species. In order to confirm this morphological identification, DNA of the plankton was extracted and PCR was performed. The sequence of the sample was compared with the sequences of the similar species from GenBank. Phylogeny and molecular analysis confirmed this identification. The present study revealed that D. salina from the south coast of Iran was able to produce resting cyst.

Neritas are among the most dominant groups of Gastropoda in the Persian Gulf. There is no previous study in relation to molecular and phylogeny of Neritas in the study area. The molecular identification of Nerita species have been studied for the first time in the northern rocky coastal zones of Persian Gulf during 2013 and 2014. After morphological identifications of species, DNA extraction, amplifying partial of cytochrome oxidase COI and 16S rRNA and sequencing procedure were done in the laboratory. In this study, 6 COI and 6 16S rRNA sequences, belonging to 3 species, were obtained. Also, phylogeny analyses with drawing phylogeny trees of Maximum Likelihood and Bayesian were done using MEGA6 and BEAST softwares. Morphological and molecular identification results were similar for 2 species and dissimilar for one species. This discrepancy shows that a combination of morphological and molecular studies is more reliable for species identification than either of them solely. The results also showed that Nerita species are monophyletic.

In order to assess the phylogenetic relationships of three rare species belonging to tribe Parablenniini was carried out, along the Coast of Oman Sea. Based on the type of habitats the specimens of Blenniid were collected from three sampling sites in the intertidal zones by Scoop and hand net in low tide and in sub-tidal zones by scuba diving and hand from 3, 6, 9, and 12m depths in September to March 2013. Totally 30 fish samples collected and deposited to the lab for further investigation. Based on the available identification keys and morphometric characters three species of Parablennius cornutus, Parabelennius pilicorn, Scartella emarginata were identified. DNA was extracted from the basal tissue of the first dorsal fin, subsequently by using a specific fish primer 700 bp of Cytochrome Oxidase C subunit 1 (CO1)was amplified in polymerase chain reaction (PCR) and sequenced. After comparison of Iranian Blenniid species CO1 sequence with sequence of 25 species from GenBank, was concluded that Iranian species are paraphyletically in the clade Parablenniini and sub-clads of Scartella and Parablennius. Since the relationship of Parablennius with other blenniids is the subject of many studies to resolve the conflicts and ambiguities among them we selected these genera to investigate the sister group relationship among them as well as other genera and clads. This is the first time that morphologic and molecular study of the two genera Parablennius and Scartella have been carried out in Iran.

Radula as the main feeding organ in gastropods not only functions in obtaining food, but also considered as an important organ in classification and phylogenic studies. In this research, the radula morphology of the two species, Conomurex persicus (Strombidae) and Mauritia grayana(Cypraeidae) from BandareLengeh and the Larak Island were analyzed. The collected samples were fixed in 70% alcohol, and radulae were isolated and processed in the laboratory, then photographed with a scanning electron microscope (SEM). Radula in these two species is of taenioglossan type with 7 teeth per row, but structure of radula teeth in two species are different from each other. Due to various type of feeding habits in C. expersicus and M. grayana, they require radulae with structure to suit their needs. On other hand, any radula structure can also imply special dietary habit, so it can be used to understand the feeding habit of a particular gastropod species.