جستجوی مقالات مرتبط با کلیدواژه « AMP » در نشریات گروه « پزشکی »

Despite the improvements in hospital care and the introduction of a wide range of antimicrobial agents, Pseudomonas aeruginosa is also a common cause of infection in patients hospitalized in different wards of the hospital. Due to the rising resistance of this bacterium to antibacterial drugs, the importance of its resistance is increased. The enzyme β-lactamase AMP-C is a type of cephalosporinase coded on the chromosome of the bacterium. In many bacteria, induction of AMP-C enzymes can occur at high levels by many mutations. In this paper, the detection of AMP-C gene was reported in clinical isolates of Pseudomonas aeruginosa.
80 Pseudomonas aeruginosa bacteria, verified by gram stain and biochemical tests, were isolated from wound samples collected from patients with burning at burning hospital in Tehran. For identifying antibiotic resistance, in vitro susceptibility of 80 Pseudomonas aeruginosa isolates to 15 antimicrobial agents, includimg colistin, amoxicillin, ceftriaxone, cefixime, cefalotin, ciprofloxacin, amikacin, doxycyclin, ampicillin, trimethoprim, ceftazidime, gentamicin, cefotaxime, piperacilin and imipenem was performed by Kirby-Bauer’s disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI, 2016) guideline. PCR method was used to identify AMP-C gene in 80 Pseudomonas aeruginosa strains.
Pseudomonas aeruginosa strains showed the highest resistance to cephalotin (100%), doxycycline (100%), cefixime (100%), amoxicillin (100%), ampicillin (100%), amikacin (100%) and teri-methaprime (100%). Highest sensitivity of Pseudomonas aeruginosa strains was observed to colistin (67.5%), gentamicin (32.5%), piperacillin (30%), ciprofloxacin (28.7%), imipenem (18.7%), ceftazidime (13.7%), ceftriaxone (11.2%) and cefotaxime (10%). AMP-C gene was detected in 5% of isolates.
According to the results of this study, in many Pseudomonas aeruginosa strains, the presence of all resistance genes in an isolate was not observed. Therefore, it can be hoped that β-lactamase enzymes are not coded in most isolates

Diabetes mellitus is associated with changes in testicular tissue. Here, the effects of co-administration of zinc and vanadium sulfate on testicular damage in diabetic animals were studied.
Moderate diabetic hyperglycemia was induced by injection of streptozotocin (STZ) 40 mg/kg, ip. Five groups of animals were selected (n=5). Group I: As normal group, during the trial period did not receive any drug treatment and consumed tap water during 45 days. Group II: As control diabetic group, received STZ 40mg/kg, ip injection. During the trial period they did not receive any drug treatment and they consumed tap water. The diabetic animals (STZ 40mg/kg, ip) with 500-600 mg/dl blood glucose levels were randomly divided into three groups: Group III: As under treatment 1, used drinkable water containing 1mg/ml of vanadium sulfate Group IV: As under treatment 2, used drinkable water (0.25 mg/ml zinc sulfate) Group V: As under treatment 3, used drinkable water (1mg/ml of vanadium sulfate and 0.25 mg/ml zinc sulfate). Testicular damage were assessed using H & E staining.
Zinc sulfate and vanadium sulfate and their combination have reduced glucose plasma levels in diabetic rats. Vanadium sulfate reduced spermatogenesis and seminiferous tubule diameter in diabetic rats. Seminiferous tubule diameter and spermatogenesis have increased in diabetic rats receiving a combination of oral zinc and vanadium sulfate.
This study demonstrated that the use of zinc sulfate reduced testicular damage and can neutralize the negative effects of vanadium sulfate on testis in diabetic rats.

Thalassemia is an inherited autosomal recessive anemia caused by structural variations or abnormalities in globin chain synthesis. The screening of thalassemia in marriage candidates is a suitable opportunity for prevention and control of this disease. Regarding hematologic indices variations in above-mentioned patients, this study was conducted to compare the hematological indices variations in types of Thalassemia in marriage candidate patients in Kohgiluye & Boyerahmad province in 1392.
In this cross-sectional study, the results of the genetic analysis by GAP-PCR method and hematological indices including MCV, MCH, HGA2 and RBC of 111 thalassemic marriage candidates who referred to health centers, along with their demographic data was analysed with descriptive statistics and inferential statistics Anova and pirson"s correlation, by SPSS 17 software.
Alpha thalassemia (78/4 percent), non-alpha-beta thalassemia (9 percent) and beta thalassemia (8.1 percent) were the most frequent types, respectively. The average of hematological indices were 2/77 ± 0/75, 75/62 ± 5/58, 23/87 ± 2/40 5/61 ± 0/62 for HbA2, MCV, MCH and RBC, respectively. Alpha thalassemia patients showed higher MCV average in comparison to beta thalassemia cases. The difference of average of MCV between patients with alpha-thalassemia and patients with non-alpha/beta was not statistically significant. MCV average in Beta thalassemia was not different with both Alpha thalassemia and non-alpha/beta thalassemia. MCH and HgA2 difference between different thalassemia types was matched with MCV but RBC average difference was not significant for any types of thalassemia. There was a direct significant correlation between MCV and MCH for all types of thalassemia. MCV and MCH indices averages have inversely correlated with RBC In patients with alpha thalassemia (p =0/01).
Ddifference of average of blood parameters including MCV, MCH and HgA2 between beta thalassemia cases with alpha, non-alpha-beta thalassemia patients with beta thalassemia cases, and beta thalassemia with both alpha and non-alpha-beta thalassemia can be used as a predictive marker for clinical diagnosis types of thalassemia among Kohgiluye and Boyerahmad province patients.

.Thyroid gland has an important role in growh and metabolism in the body. Hypothyroidism is a disorder that act on body organs. The medicinal plants in treatment of disease are widely used. Elettaria Cardamomum L. is known as a medicinal plant with widely use in traditional medicine. In this study the Elettaria Cardamomum hydroethanolic extract (ECE) on hypothyroid male mice has been investigated .
Material and In this experimental study 42 male mice were divided to 6 groups randomly: control groups, witness (taking propylthiouracil ,PTU 0.1% in tap water), treated groups 1,2 and 3: (taking PTU, 0.1% in tap water 100, 200 and 400 mg/kg ECE , i.p) and levothyroxine group (taking PTU, 0.1% in tap water 15 µg/kg. gavaged). The PTU 0.1% administered for 15 days, then the groups received ECE and levothyroxine for 10 days. The control group were received daily 0.25ml normal saline intraperituneally. At the end of experiment, animals were anesthetized by ether and blood samples collected from heart directly for analyzing TSH,T3 & T4 hormones. The data were expressed as mean±SEM and analyzed by ANOVA statistical software. The significant differences were expressed with P The results of this study showed that the T3 and T4 level decreased and TSH level increased compared with control group significantly in taking PTU group(P The ECE may be has antioxidant and flavonoid compounds which are able to act on thyroid hormones synthesis considerably and increase its serum levels in male mice.

Spatial distribution of cancer is one of the powerful tools in epidemiology of cancer. The present study is designed to understand the geographical distribution of most frequent types of cancer in K&B province.
All registered cases of cancer are reviewed and duplicate cases were removed. The data was analyzed using Arcgis software.
Of all registered cases, 1273 remained for analysis of which 57% were residences of urban areas. Cities including Sisakht, Yasuj and Dehdsasht were shown to have highest incidence rates among the Urban areas. Dena, Sepidar and Kohmare Khaleghi had the highest rates among the rural areas in the province. Skin cancer was the most common type of cancer which had the highest rates of incidence in Sisakht and Dehdasht and Dena and Sepidar among urban and rural areas respectively.
The distribution of cancer was not even in the province. Attitude and consumption of wild and regional plants are introduced as the potential risk factors for such a spatial distribution of the common cancers I the province. The results of this study could be used for further analytical studies to understand the regional etiology of cancer in the province.

Background and An increasing emergence of multidrug resistance among Klebsiella pneumoniae nosocomial isolates has limited the therapeutic options for treatment. The beta-lactamases are the major defense of gram-negative bacteria against antibiotics. The aim of this study was the detection of ESBLs and Amp-C enzymes and MOX، FOX، and ACT genes in K. pneumoniae strains isolated from hospitalized patients in Tehran hospitals during 2011-2013. This study was conducted in 120 K. pneumoniae isolates from Imam Hossein، Taleghani and Mofid Children''s hospitals in Tehran، Iran. Antibiotic susceptibility tests were performed using Kirby-Bauer disc diffusion and Broth Microdilution methods. The detection of Amp-C and ESBLs enzymes was carried out according to CLSI guidelines. The MOX، FOX، and ACT genes were detected by PCR and sequencing methods. Among 120 K. pneumoniae strains، 24 (20%) and 68 (56. 8%) were Amp-C and ESBL positive، respectively. In this study colistin and tigecycline were found more active than other antibiotics. ACT، FOX، and MOX genes were detected in 16 (13%)، 46 (38. 33%) and 43 (35. 83%) of the isolates، respectively. The prevalence of antibiotic resistance genes detected in this study is of great concern and highlights the need for infection control measures including antibacterial management and identification of resistant isolates.

Considering the role of human errors in the incidence of catastrophic events in control rooms and also Lack of effectiveness of classical techniques to identify the human errors، special techniques are required for identification of human errors. Therefore، this study aimed to identify human errors in the control room in an oil company Using HEIST Technique. The present study is a case study of qualitative research that was conducted with using HEIST Technique. Collection of the required information has been done with Walking-Talking Trough and «Rose & Roses» model that is used for classification the decision-making process. «Kirwan» model is used for classification of factors that are involved in human errors and Types of errors have been detected with the SRK Model. Overall، 300 human errors has been detected. Of which،» interactions with controllers and indicators «،» instructions «and» training and experience «alone causes 71 percent of human errors in control room operators. In addition، 90% of human errors were related to the «stages of implementation of the solution»، «observing system» and «selection hypothesis». The results of this study showed a variety of possible scenarios resulting from human errors and their unintended consequences. These results emphasize that there are several weaknesses suggesting the implementation of both engineering and administrative controls simultaneously to reduce human errors

Identifying factors affecting academic achievement and attention to them is an important step in improving educational quality. Awareness of students’ viewpoints, especially talented and outstanding is very useful and effective. This study conducted to determine factors affecting the success and achievement of talent students from their viewpoints. In this descriptive study, 38 outstanding and talented students in Rafsanjan Medical University completed a researcher-made questionnaire which has been designed based on Herzberg’s theory. The validity and reliability of the questionnaire was confirmed by content validity and test re-tests. The data was analyzed by using t- and Chi-square tests. The finding showed the most important factors affecting the academic achievement were as follow: regular participation and presence in classroom (84.3%), tendency for continuing academic studies (81.6%), interesting to academic major (79%), encouraging family and friends (76.3%), and ability to communicate with instructors (73.6%). According to points of view of excellent students some factors especially personal characters affected their academic achievement. These factors should be considered to promote educational quality.