جستجوی مقالات مرتبط با کلیدواژه « Bacterial » در نشریات گروه « پزشکی »

The use of plant extracts or their compounds as antimicrobial agents for oral infections worldwide represents that herbal medicines could be used as an effective alternative method in oral health care. This study aimed to evaluate the antifungal and antibacterial effects of five traditional medicinal plant extracts on standard and clinical strains of bacteria and fungi causing dental caries.

Aqueous and methanolic extracts of Zataria multiflora, Lawsonia alba, Zizyphus spina-christi, Myrtus communis, and Citrus aurantium were prepared using maceration method. The minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) of the prepared extracts were evaluated against bacterial (Streptococcus sanguinis and S. mutans) and fungal (Candida albicans, C. krusei, and C. tropicalis) isolates using broth microdilution method.  

Aqueous extracts of the studied plants showed no antimicrobial effects on the studied microorganisms, except M. communis and C. aurantium. The results indicated the antimicrobial potency of the methanolic extract of M. communis (MIC range =2 to 64 µg/mL) against all the studied microorganisms, followed by Z. multiflora (MIC range = 512-2048 µg/mL), L. alba (MIC range = 1024-2048 µg/mL), C. aurantium (MIC range = 1024-4096 µg/mL), and Z. spina-christi (MIC range=2048- ˃4096 μg/mL).  Also, the lowest MMCs against the studied strains were related to the methanolic extract of M. communis (MMC range = 16-512 µg/mL).

The results showed remarkable antimicrobial effects of M. communis extract, which could be a suitable alternative to chemical mouthwashes to prevent and control oral infections.

Otomycosis is a common fungal ear infection affecting people worldwide. Cases may be exacerbated by mixed fungal-bacterial infections, especially those involving antibiotic-resistant bacteria. Understanding the microbiological features and antibiotic susceptibility patterns of the pathogens involved is critical for treatment. This study aimed to investigate the prevalence of mixed fungal-bacterial infections in otomycosis cases in Indonesia, to identify the bacterial species involved, and to determine their antibiotic susceptibility patterns.

In this study, 47 ear swab specimens were collected from 41 clinically-diagnosed otomycosis cases from April to August 2022. The collected samples were processed by culture and microscopy to identify fungal and bacterial isolates. Antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method.

Fungal isolates alone were detected in 80.9% of the specimens, while 19.1% showed mixed fungal-bacterial growth. The most common fungi were Aspergillus (57.1%) and Candida (42.9%) species. Among bacterial isolates, Staphylococcus aureus was the most frequent (observed in 66.7% of mixed cases), followed by Pseudomonas aeruginosa (22.2%). Also, two of the six S. aureus isolates were methicillin-resistant (MRSA). Both P. aeruginosa isolates were extended-spectrum beta-lactamase (ESBL) producers.

Many otomycosis cases in this study demonstrated polymicrobial etiology. The emergence of antibiotic-resistant bacteria poses diagnostic and therapeutic challenges to healthcare systems.

Antibiotic resistance is a significant problem that restricts the options for treating bacterial pneumonia. This research aimed to determine the bacterial causes of pneumonia and antibiotic resistance among hospitalized patients in southwest Ethiopia.

We collected and analyzed 150 sputum samples from individuals with community-acquired pneumonia from April 1st to October 30th, 2019. Standard bacteriological procedures were used to identify the bacteria. Kirby Bauer's disk diffusion method was used to assess the bacteria's susceptibility patterns. Production of carbapenemase and extended-spectrum-lactamase were confirmed phenotypically. Odds ratios and the chi-square test were computed.

On the whole, bacterial pathogens were verified in 50% of the sputum samples. The predominant bacterial isolates were Klebsiella species, followed by Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pneumoniae. About 77.5% of isolates were multidrug resistant. Moreover, 40.5% and 10.8% of the isolates were ESBL and carbapenemase producers, respectively. Aging, tobacco smoking, previous history of pneumonia, heart disease, and chronic respiratory disease had association with sputum culture-positivity.

As a result, it is important to regularly monitor the bacterial etiologies and their patterns of resistance. Additionally, sociodemographic and clinical characteristics should all be taken into account while managing patients with pneumonia empirically in this context.

The study aimed to investigate whether Achromobacter mucicolens IA strain biofilm forma- tion, which contributes to antibiotic resistance, could be enhanced by readily available nutrient sources like carbohydrates and environmental factors such as pH and NaCl. Additionally, the study aimed to identify any inherent genes that support biofilm formation in this strain, which is an opportunistic pathogen that affects immunocompromised patients and is resistant to many antibiotics.

Biofilm growth in different carbohydrate, pH, and NaCl concentrated media was measured using crystal violet microtiter assay. All the treatments were subjected to biostatistics analysis for normality, Test of Homogeneity, one way ANOVA analysis. Whole-genome sequencing of our IA strain was conducted to identify various gene sequences.

Biofilm formation was measured at different carbohydrate concentrations, and the optimum biofilm formation was observed at 3M glucose and 0.5M NaCl, while the lowest results were seen at 2M maltose concentration. Whole-genome sequencing identified potential genes involved in biofilm formation, pathogenicity, protein metabolism, flagellar motility, cell wall component synthesis, and a multidrug efflux pump.

These findings suggest that biofilm formation is influenced by extrinsic and intrinsic factors, which could aid in the development of effective treatments for resistant infections.

Abnormal vaginal discharge is a common problem among pregnant women. The most common cause of these discharges is bacterial vaginosis (BV), which has numerous complications and causes problems for pregnant mothers and their fetuses. The purpose of this study was to determine the BV frequency among pregnant women referring to a gynecology clinic in Arak city using Amsel and Nugent criteria, Alberta guideline, and PCR.

This descriptive study was performed on 70 vaginal samples of pregnant women in Arak to investigate the most common causes of vaginal discharge according to Amsel and Nugent criteria and polymerase chain reaction (PCR) method using specific primers targeted towards three bacteria: Gardnerella vaginalis, Atopobium vaginae, Mobiluncus curtisii. Data were analyzed using SPSS software and Chi-square test.

In this study, ten (14.28%) out of 70 pregnant women had positive bacterial vaginosis according to Amsel criteria. According to Nugent criteria and Alberta guideline, three (4.29%) cases were diagnosed with definite BV, 20 (32.26%) cases with intermediate BV with clue cells, 42 (67.74%) cases with intermediate BV without clue cells, and finally five (4.29%) cases with negative BV. Also, according to PCR, the frequency of G. vaginalis, M. curtisii, and A. vaginae in vaginal samples was 71.42% (50 cases), 64.28% (45 cases), and 30% (21 cases), respectively. 

According to the obtained results, the prevalence of definite bacterial vaginosis was lower than that of vaginitis, and most patients suffered from nonspecific vaginitis.

We aimed to elucidate the pathogenic bacterial and fungal profiles of specimens obtained from suspected ocular infections at Farabi Eye Tertiary Referral Hospital, Tehran, Iran.

In this cross-sectional study, we collected data from ocular specimens taken during the seven-year period of 2011 to 2018, and the results were then retrospectively analyzed. Samples had been obtained from patients who were investigated for ocular infections.

Overall, 16,656 ocular samples were evaluated. The mean patient age was 48.31 ± 26.62 years. Most patients were men (60.33%), and men in the 7th decade of life were the largest represented group. The seasonal distributions of specimen collection sites followed the overall distribution of collection sites by year. Specimens obtained from the cornea were the most common (49.24%), also representing the largest number of specimens in all seasons. The most commonly isolated fungal microorganisms were Fusarium spp., followed by Aspergillus spp. and Candida albicans. Of the 6,556 specimens with positive bacterial cultures, 59% produced gram-positive bacteria, while the remainder produced gram-negative pathogens. The most commonly isolated bacteria were Pseudomonas aeruginosa (17.77%), Staphylococcus epidermidis (13.80%), Streptococcus pneumoniae (13.27%), S. viridans (12.23%), and S. aureus (11.18%).

Most submitted specimens were obtained from the cornea. The most commonly isolated fungal microorganisms were Fusarium spp., followed by Aspergillus spp. and C. albicans. The most commonly isolated bacteria were P. aeruginosa, followed by S. epidermidis and S. pneumoniae.

The term "Nosocomial" is attributed to the diseases acquired by the patient under medical care. Various microorganisms, including bacteria, viruses, and fungi, may contribute to developing nosocomial infections (NIs). Urinary tract infections (UTI), surgical-site infections (SSI), bloodstream infections (BSI), and pneumonia are the most well-known instances. We investigated various aspects of NIs and the main causative agents of NIs, particularly bacteria, antibiotic resistance, crucial viral infections in hospitals, and a brief survey of fungal infections. It was concluded that specific human body tissues such as those in the lungs and urinary tract are more likely to be a target for nosocomial pathogens. The fatalities associated with these infections, particularly in the intensive care unit (ICU), are serious concerns, and transmission by health facilities has become a primary medical issue because of its spread into the community. Another medical point is antibiotic resistance which is a leading cause of prolonged periods of hospitalization and makes the treatment procedure harder and costlier. Additionally, measures to prevent the spread of NIs and minimize the economic loss are discussed. All physicians and medical students must be updated about different kinds of these infections, their causative agents, challenges, and how to deal with them to reduce the consequences and improve public health.

The prevalence of nontuberculous mycobacteria (NTM) infection has been increasing globally. Many cases of NTM infection are misdiagnosed as Mycobacterium tuberculous (MTB) because of similar clinicoradiological features.

To determine the burden and characteristics of NTM infection, this study was done to evaluate clinical isolates collected from tuberculous (TB) suspects in a population from Northwest China.

From January to December 2020, the clinical samples of 9,142 TB suspects were collected for the PCR-fluorescent probe and mycobacterial culture. The PCR-fluorescent probe-positive nucleic acid samples were further subjected to a DNA microarray for confirmation and species identification. Drug susceptibility testing (DST) was also carried out using the micropore plate method (MicroDSTTM) on isolates from NTM patients.

Of 9,412 TB suspects, 85 cases (0.9%) were clinically diagnosed with NTM infection according to the American Thoracic Society (ATS) guidelines. For the laboratory samples, a total of 169 NTM strains, identified by molecular biology methods, were classified into 10 species. Themost common species were M. chelonae/ M. abscessus (64/169, 37.7%) and M. intracellulare (40/169, 23.7%). All strains showed the highest resistance to imipenem/cilastatin (85/85, 100%) and the highest susceptibility to linezolid (4/85, 4.7%). In comparison with the rapidly growing mycobacteria (RGM) group, the slowly growing mycobacteria (SGM) group showed a lower resistance and a shorter hospital inpatient stay (t = 6.66, P < 0.001 and t = 2.40, P = 0.020, respectively).

Mycobacterium chelonae/M. abscessus and M. intracellulare were the most frequently detected NTM pathogens in Northwest China. The differences in drug sensitivity and clinical characteristics were giant for different strains. Timely identification and accurate DST play important roles in NTM management.

Skin and soft tissue infections are important causes of outpatient visits to medical clinics or hos-pitals. This study aimed to review the literature for the accuracy of Clinical Resource Efficiency Support Team(CREST) guideline in management of cellulitis in emergency department.

Studies that had evaluatedcellulitis patients using the CREST guideline were quarried in Scopus, Web of Science, and PubMed database,from 2005 to the end of 2020. The quality of the studies was evaluated using Scottish Intercollegiate GuidelineNetwork (SIGN) checklist for cohort studies. Pooled area under the receiver operating characteristic curve (AU-ROC) of CREST guideline regarding the rate of hospital stay more than 24 hours, rate of revisit, and appropriate-ness of antimicrobial treatment in management of cellulitis in emergency department was evaluated.

Seven studies evaluating a total of 1640 adult cellulitis patients were finally entered to the study. In evaluation ofthe rate of the appropriate treatment versus over-treatment, the pooled AUROC was estimated to be 0.38 (95%confidence interval (CI): 0.06 – 0.82), indicating low accuracy (AUROC lower than 0.5) of guideline for antimicro-bial choice. CREST II patients had a significantly lower odds ratio (OR) of revisiting the Emergency Department,OR=0.21 (95% CI: 0.009 – 0.47). Pooled AUROC value of 0.86 (CI95%: 0.84 – 0.89) showed accuracy of the CRESTclassification in prediction of being hospitalized more or less than 24 hours.

CREST classificationshows good accuracy in determining the duration of hospitalization or observation in ED but it could lead toinevitable over/under treatment with empirical antimicrobial agents.

The aim of this study was to investigate the density and type of bacterial and fungal bioaerosols in the air of the pediatric burn ward.

In this cross-sectional study, two active and passive sampling methods were used simultaneously to evaluate the density and type of bacterial and fungal bioaerosols. In 2019, sampling was performed once every six days, according to the sampling guideline developed by the 2019 United States Environmental Protection Agency (EPA). Data were analyzed using SPSS software (version 22.0).

According to the EU GMP standard, in the active method, bacterial and fungal contaminations in the indoor air of the burn ward were in grades C and D, respectively. According to this standard, in the active method, bacterial and fungal contaminations in the outdoor air of the burn ward were in grade C. According to the EU GMP standard, in the passive method, bacterial and fungal contaminations in the indoor air of the pediatric burn ward were in grade C. According to this standard, in the passive method, bacterial and fungal contaminations in the outdoor air of the burn ward were in grade C.

Given the importance of preventing infection in patients with burns and preventing deaths caused by infections in these patients, especially in children with burns, it is necessary to pay attention to the role of bioaerosols in developing nosocomial infections in burn patients.

To determine if there is a difference in the quantity of microbial flora of the conjunctiva in individuals practicing head submersion (“dunk”) versus no head submersion(“no-dunk”) during hot tub use.

In this double-blind randomized clinical trial, healthy volunteers aged ≥ 18 years were recruited. Participants were randomized to head submersion versus no head submersion during a 15-minute hot tub soak. Study personnel,masked to the dunkor no-dunk group assignment,obtained conjunctival cultures before and immediately after hot tub use. De-identified specimens were submitted to the clinical microbiology laboratory for culture and analysis. The main outcome measure was the difference in the quantity of organisms cultured from the conjunctiva beforeand after hot tub exposure, as determined usinga defined ordinal scale.A two-tailed Student’s t-test was performed to compare the total microbial colony counts between the two arms. Simpson’s diversity was used to measure the changes in organism diversity between the arms.

Of 36 enrolled subjects, 19 were randomly assigned to thedunkand 17 were assigned to the no-dunk groups. Water samples obtained from all hot tubs wereculture negative. Eleven of 19 eyes (58%) from the dunkgroup and eight of 17 eyes (47%) from the no-dunkgroup had negative conjunctivalbacterial cultures before and after hot tub exposure. However, six of 19 eyes (32%) and four of 17 eyes (24%) of the dunkand no-dunkgroups, respectively, were culture-positiveafter,but not beforehot tub exposure. The quantity of organisms before and after hot tub exposure was notsignificantlydifferentbetween the two arms (P=0.12). However, the dunk grouponly showed a small increase in the quantity of organisms afterascompared to beforehot tub use(P=0.03). None of the samples from subjects or hot tubs were culture-positive for Acanthamoeba.

Head submersion in a public hot tubsduring a 15-minute soak does not appear to change conjunctival flora,as determinedby culture plate yield, this does not eliminate the association between hot tub use and devastating and painful corneal blindness. Therefore, our recommendation is to remove contact lenses prior to hot tub use, avoid head submersion in a hot tub, and urgently seek ophthalmological help if any eye pain and/or decrease in vision is experienced after hot tub use.

Conjunctivitis is a commonly encountered condition in ophthalmology clinics throughout the world. In the management of suspected cases of conjunctivitis, alarming signs for more serious intraocular conditions, such as severe pain, decreased vision, and painful pupillary reaction, must be considered. Additionally, a thorough medical and ophthalmic history should be obtained and a thorough physical examination should be done in patients with atypical findings and chronic course. Concurrent physical exam findings with relevant history may reveal the presence of a systemic condition with involvement of the conjunctiva. Viral conjunctivitis remains to be the most common overall cause of conjunctivitis. Bacterial conjunctivitis is encountered less frequently and it is the second most common cause of infectious conjunctivitis. Allergic conjunctivitis is encountered in nearly half of the population and the findings include itching, mucoid discharge, chemosis, and eyelid edema. Long-term usage of eye drops with preservatives in a patient with conjunctival irritation and discharge points to the toxic conjunctivitis as the underlying etiology. Effective management of conjunctivitis includes timely diagnosis, appropriate differentiation of the various etiologies, and appropriate treatment.

Nosocomial infections are associated with increased morbidity, mortality, and medical burdens. Pseudomonas aeruginosa and Acinetobacter baumannii are not-fermentative gram-negative bacteria that considered as the most important nosocomial infection. In the current study, we have aimed to evaluate the sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa microorganisms to the colistin antibiotic.

In this descriptive cross-sectional study, patients admitted to the ICU ward of Firoozgar Hospital from July 2018 to March 2019 were evaluated, and 169 Patients infected with Acinetobacter baumannii, and Pseudomonas aeruginosa were included. Acinetobacter baumannii and Pseudomonas aeruginosa were isolated, and antibiotic sensitivity was determined by the disk diffusion method according to Clinical & Laboratory Standards Institute (CLSI) criteria. E test was also used to determine MIC-50 and MIC-90 of colistin.

Acinetobacter baumannii was around 8 times more frequent than Pseudomonas aeruginosa. Colistin resistance was detected in only 4(2.4%). The mean age of patients infected by Acinetobacter baumannii was significantly higher than those infected with Pseudomonas aeruginosa. Moreover, the mean time of the hospitalization period did not show any significant differences in the different groups.

Our findings indicated that the majority of isolated Pseudomonas aeruginosa and Acinetobacter baumannii were sensitive to Colistin. Therefore, it could be effectively used for patients with a confirmed diagnosis of Pseudomonas aeruginosa and Acinetobacter baumannii.

Vancomycin is a potent antibiotic and has central role in the managing of infections with known resistance to other antibiotics or in patients with allergy to beta-lactams. Irrational use of vancomycin is associated with increased morbidity and mortality as well as the antibiotic resistant.

The DUE was done in Imam Reza Hospital, Tabriz, Iran. A total of 100 patients were included during a 6-month period. We aimed to evaluate vancomycin administration pattern and assess its compliance with Centers for Disease Control and Prevention (CDC) and the American Society of Health-System Pharmacists (ASHP) protocols as the primary outcome and its adverse effects as the secondary outcome.

The mean duration of hospitalization and antibiotic therapy were 22.11 ± 1.76 and 19.08 ± 1.51 respectively (mean ± SD). The most causes of vancomycin administration (51%) were for surgery prophylaxis. In 38% of patients, vancomycin administration was not in accordance to standard guidelines. Dose and duration of vancomycin therapy was according to ASHP and CDC guidelines in 74% and 59% of patients. Dose readjustments of antibiotics were necessary in 28 patients which were done in 12 of them. A total of 140 samples were collected from 60 patients. In 30% of patients, vancomycin use was continued without considering the culture results.

It is important to set practical pharmaceutical and therapeutic infection control committees in hospitals under the clinical pharmacists’ observation. Furthermore, educational programs for health care professionals regarding rational use of antibiotics can be helpful in improving antimicrobial medications utilization and monitoring.

Extended‑spectrum ß‑lactamase (ESBL)‑producing Enterobacteriaceae seem to have an extended antibiotic resistance,but have different resistance patterns throughout different sites and regions.This study aimed to evaluate the antibiotic resistance pattern of ESBL‑producing Escherichia coli.

One hundred swab samples from patients hospitalized due to a clinical suspicion of any kind of infection (with manifestations such as fever, leukocytosis, and an active urinalysis result) were processed in Alzahra Microbiology Laboratory, Isfahan, Iran. Isolated E. coli were cultured on Mueller–Hinton agar and antibiotic susceptibility was tested by Kirby–Bauer disk diffusion method following the Clinical and Laboratory Standard Institute 2017 guidelines.

ESBL‑producing samples had higher antibiotic resistance rates than ESBL‑non‑producing samples: ceftriaxone (58.8% vs. 27.3%), cefotaxime (73.5% vs. 30.3%), ceftizoxime (76.5% vs. 33.3%), cefixime (79.4% vs. 40.9%), and cefpodoxime (73.5% vs. 53%), except for carbenicillin (29.4% vs. 48.5%). Imipenem and meropenem were the least resisted antibiotics in ESBL‑producing samples (5.9% and 11.8%).

ESBL‑producing Enterobacteriaceae have a high resistance rate to third‑generation cephalosporins and high susceptibility to imipenem and meropenem.

Ear infection is one of the most common types of infections that can caused by fungal and bacterial agents. The precise diagnosis of the disease and identification of microbial agents is very important to prescribe the right drug and cure patients as soon as possible. The present study was to find the consistency of clinical findings and laboratory tests among patients suspected of ear infections over a one-year period. In this cross-sectional study conducted in 2018 to 2019, 134 ear samples of patients referred to the ENT clinic of Ayatollah Rouhani hospital of Babol were collected. These samples were analyzed and microbial agents were identified by direct examination and culture. The results were compared with an initial diagnosis from a physician. Out of the 84 patients clinically diagnosed for fungal infection, 67 cases (79.8%) were laboratory-approved, while the rate of bacterial- infection was 33 cases (66%). Our findings showed that the initial diagnosis of from a physician along with laboratory tests is necessary for accurate diagnosis and treatment of ear infections.