جستجوی مقالات مرتبط با کلیدواژه « Bovine ephemeral fever virus (BEFV) » در نشریات گروه « دامپزشکی »

The G glycoprotein of bovine ephemeral fever virus (BEFV) has been identified as a plausible candidate for immunization against BEF disease. In recent years, this protein has been investigated to produce a recombinant vaccine. The aim of the present study was to construct a eukaryotic plasmid, expressing G1 epitope of BEFV G glycoprotein gene, for application as a possible DNA vaccine in future studies. For this purpose, the G1 epitope of G glycoprotein gene was cloned in a eukaryotic expression vector, pEGFP-N1, under the control of the human cytomegalovirus (CMV) promoter. Then, the recombinant pEGFPN1-G1 construct was transfected into human embryonic kidney (HEK 293) cell line and the expression efficiency was verified by indirect immunofluorescent (IFA) staining and immunoblotting techniques. Observation of intracytoplasmic fluorescence in transfected cells and appearance of a distinct band at an approximate molecular weight of 26 kDa in immunoblotting in reaction to an anti-G1 mouse serum, indicated that G1 protein was successfully expressed by this recombinant construct in the host cells.

Bovine ephemeral fever (BEF) is a non contagious disease of cattle and water buffalo, spanning in tropical and subtropical zones such as Asia, Australia, and Africa. The aim of this study was to investigate the reactivity of the fusion MBP-G1 protein expressed in Escherichia coli in an indirect in-house ELISA. In order to, after cloning of the G1 gene of bovine ephemeral fever virus (BEFV) G glycoprotein into pMalc2x vector and subsequently expression in Rosetta strain of Escherichia coli, recombinant MBP-G1 proteinwasused as a coating antigen to develop an ELISA. Results showed that the expressed G1 protein could react with immunized mice serum with commercial BEF vaccine and detect of antibodies against BEFV. Thus, the expressed fusion MBP-G1 protein can be introduced as an ideal candidate to develop an ELISA for detection of BEFV-infected water buffalo.