مجله سازمان نظام پزشکی جمهوری اسلامی ایران
سال سی و چهارم شماره 3 (پیاپی 135، پاییز 1395)

For the evaluation of the health status different questionnaires were used. The World Health Survey (WHS) Individual questionnaire has been applied in different countries as a short and standard tool. This questionnaire measures the health status of the individual in 9 domains. The objective of the current study was to validate the WHSIndividual questionnaire in Farsi.
In this validation study,forward-backward translation procedure was applied to the questionnaire. The validity of the questionnaire was assessed by 10 content and methodology experts and somepotential participants.Inter Rater Agreement (IRA), relevancy and clarity foreach item as well as comprehensiveness of the questionnairewere calculated.Reliability was measured usingCronbach`s alpha and Intra Class Correlation coefficient (ICC) through test-re-test in 20 convenient samples. To assess the discriminant validity, meanscoreswere compared between people with different self-rated health (SRH) status (good SRH=1743people and bad SRH=183).The means scores of the current questionnaire were also compared in two groups regarding the objective health (healthy=991, unhealthy=923).
Findings: Total clarity, relevancy and comprehensiveness of the questionnairewere 91%, 94% and 90%, respectively. ICC ranged between 0.68 and 1 with the median of 0.89. Chronbach`s Alpha was 0.76. The average scores in groups with good and bad self ­­-rated health were 14.3±13.2 and 41.9±17.9, respectively (p This study has provided some preliminary evidence on the reliability and validity of the Iranian version of the World Health Survey Individual Questionnaire.

Anxiety is the most common physiological and psychological response to pain in burn patients, which can affect their physical and mental performance. This study aimed to determine the association between the experience of pain (sensory stimuli and emotional component) and mortality in burned patient's anxiety.
This cross-sectional study in the burn ward of Imam Khomeini University of Medical Sciences, Kermanshah. 103 patients in burn units for women and men were randomly selected and examined. The data from questionnaires blesky (1999) the level of death anxiety and anxiety in burn patients and the McGill pain questionnaire were used to measure pain. The data collected by statistical software SPSS 18, Spearman correlation coefficient test was analyzed at the level of 5%.
Based on research findings between the experience of pain (sensory stimuli and emotional component) and the anxiety of death in the first and second weeks there is a significant relationship There is also a significant correlation between the third and fourth week. The results predict death anxiety by pain, the model can justify 6/54% of the variance And pain (stimulating sensory and emotional components) was a significant predictor for death anxiety.
According to the results of this study can be concluded that the health care and control of pain (sensory stimuli and emotional components) to control the anxiety of death in these patients burn patients should be given special attention.

Yersinia pestis, a Gram-negative, non-motile and Slow growth bacterium belonging to the Enterobacteriaceae family, is the causative agent of plague. It has been classified by CDC in group A of bioterrorism agents due to its high morbidity and mortality and easy person to person dissemination. In spite of sensitivity and High accuracy PCR, false negative results can occur due to the presence of inhibitors in clinical sample. The aim of this study was to construction an internal control for the specific detection of Y.pestis.
In this study, The diagnostic primers for the F1 capsule antigen gene (caf1) and the plasminogen activator gene (pla) were designed using Allele ID 7.6 software. our target region was a conserved fragment in the pla and caf1 genes of Y. pestis. The composit primers for IC were designed using Pichia pastoris AOX1 gene sequences and target gene. The PCR experiment was performed on genomic DNA of Y.pestis. The product was cloned in the pTZ57R/T vector to construct a internal control. Then, the internal control function was evaluated By using diagnostic primers.
Findings: The PCR products of the pla and caf1 genes were 136bp and 117bp respectively on electrophoresis gel and length of IPC were 225bp and 227bp respectively, so there was a significant different between their size.
The results indicate that the IC effective tool for avoid false negative results and confirm the results.

Medical science excellent centers have consistently sought to improve scientific status, expand the knowledge base, increase the number of expert researchers, create a dynamic environment, and promote a productive and effective international scientific network during the past several decades. In Iran since 1379, medical excellent centers joined the education and research structure as the highest scientific institutions and now there are 50 excellent centers. The senior management of the higher education system in the country is responsible for evaluation of these newly established scientific institutions. Assessment of these centers shows the quality their activities, which can contribute to quality improvement through dynamic feedback for the planning and activity development. In order to perform this assessment the Iranian Ministry of Health and Medical Education as an important part of higher education system designed a model of assessment and put it on its agenda in 2010 and was revised in 2015. This article aims to describe the process of developing the tools for needed for this evaluation. The evaluation criteria has been developed based on “input”, “process” and “educational, research, and services outcome” indexes. This evaluation has been considered as a comprehensive one because of including all aspects of management such as supply and training human resources, financing, providing equipment, process of empowerment and knowledge production as well as output in the field of education, research, industry and services. This scientific approach to the assessment and ranking of excellent centers makes them capable of being aware of their strengths and weaknesses. As a result, they can improve their scientific capacity and activities.