مجله دانشگاه علوم پزشکی قم
سال سوم شماره 4 (پیاپی 12، زمستان 1388)

LDH-C4 is one of the lactate dehydrogenase isoenzymes found in mature testis and spermatozoa of many species. The Physiological function of these isoenzymes indicates its role in creating energy for sperm motility and survival. In this research the effects of oxamate as specific inhibitors of the LDH-C4 of rat were studied in vivo. A total of 20 adult rats were divided into four equal groups. One group as the control group received saline only and different amounts of oxamate were injected into other three groups (600 300 150mg/kg) daily for 45 days intraperitoneally. The rats were then killed with chloroform and the caudal part of epididym was separated. By making several cuts in caudal part of the epididymis the sperms were isolated and put in T6 medium+5mg/ml-1 BSA. Later the sperms were incubated under 37ºC and 5% CO2 for one hour. LDH-C4 enzyme was extracted using the Erwin Goldberg and the protein amounts were measured by Lowry's method. Relative purification was done in two stages including ammonium sulfate precipitation and column chromatography by DEAE-Sephadex-A50. All stages of extraction the amount of total protein LDH enzyme activity in the oxamate-exposed groups and specified recipient were then compared with the control group. In this study the total enzyme LDH-C4 activity in the control group was 11.8±0.3 and the oxamate recipient groups (150 300 and 600mg/kg) were 8.3±0.3 6.9±0.2 and 3.2±0.1 IU respectively. The concentration of 600mg/kg oxamate was inhibited about 63% enzyme activity compared to control group. This study showed that the oxamate may reduce in vivo enzyme activity through LDH-C4 with increasing concentration and this effect is proportional. Therefore with the effect of the competitive inhibitors of oxamate on LDH-C4 this substrate can be used as a contraceptive for males. Keywords: LDH-C4; Oamate; Sperm; Rat.

Acute Effects of Apple Cider Vinegar Intake on Some Biochemical Risk Factors of Atherosclerosis in Rabbits Fed with a High Cholesterol Diet Metabolic changes in postprandial stage especially after consumption of high fat meal cause atherosclerosis and increase the risk of cardiovascular diseases. Apple cider vinegar is an acidic juice with useful medicinal effects. In this research; we investigated acute effects of apple cider vinegar intake on some of the biochemical atherosclerosis risk factors in high cholesterol fed rabbits. Thirty two male New Zealand rabbits were randomly divided into four groups: normal diet group high cholesterol diet group (%1cholesterol) %1 cholesterol with 5ml apple cider vinegar group %1 cholesterol with 10ml apple cider vinegar group. The C-Reactive Protein (CRP) low density lipoprotein (LDL-C) high density lipoprotein (HDL-C) total cholesterol (TC) malondialdehyde (MDA) oxidized-LDL (OxLDL) serum glutamic pyruvic transaminase (SGPT) serum glutamic oxaloacetate transaminase (SGOT) nitrite nitrate glucose fibrinogen triacylglycerol (TG) apolipoprotein A (ApoA1) apolipoprotein B (ApoB100) were all measured before the experiment and three hours after feeding with these treatment diets. In high cholesterol diet fibrinogen nitrite glucose OxLDL MDA and CRP showed a significant increase compared to normal diet. Significant differences were observed between both groups of apple cider vinegar by fibrinogen in comparison with hypercholesterolemic diet. Using 10ml apple cider vinegar with cholesterolemic diet caused a significant reduction in Ox-LDL MDA and glucose in comparison with hypercholesterolemic diet. Moreover the consumption of 5ml apple cider vinegar with cholesterolemic diet caused a significant decrease in LDL-C and TC compared to hypercholesterolemic diet. No significant difference was found between apple cider vinegar taking groups and hypercholesterol diet in CRP TG ApoA HDL-C ApoB SGOT SGPT nitrite and nitrate. The results showed that acute consumption of apple cider vinegar (as an antioxidant) causes significant reduction on some risk factors of atherosclerosis. Keywords: Apple Cider Vinegar; Acute Effect; Atherosclerosis. Corresponding Author: Islamic Azad University of Izeh Izeh Iran. Phone No.: 0913 312 1589 Email: doctor.setorgi@gmail.com Received: 2 May 2009 Accepted: 9 Sep 2009

Neurotrophic Effects of Silibinin on Differentiation of Hair Follicle Stem Cells to NeuronsAbstract Hair Follicle Bulge region due to its availability and abundance is one of the areas which is easily accessible to Multi-potent stem cells that expresses Nestin marker (neuronal stem cells protein). Stem cells bulge region in hair follicle stem cells has high potency to be differentiated to neuronal cells. Silibinin as an active component of Silybum marianum has anti-oxidant anti-inflammatory anti-carcinogenic hepatoprotective neurotrophic and neuroprotective effects. The aim of the present study was to evaluate the neurotrophic effects of silibinin on differentiation of hair follicle stem cells to neurons. Bulge area of whiskers in Rat was isolated and cultivated three weeks in supplemented DMEM/F12 and epidermal growth factor (EGF). Then the cells were exposed over the concentrations of 0.05μg/ml 0.1μg/ml 0.4μg/ml 0.5µg/ml 0.7µg/ml Silibinin and Neurotrophin-3. Two weeks after culture plated bulge cells were immunostained with Nestin and differentiated stem cells were immunostained with β III tubulin by immunocytochemistry techniques. The results were evaluated by T-test student analysis. A Pvalue less than 0.05 was considered significant. The nestin marker was clearly demonstrated in bulge regions during the first week but after two weeks parallel to stem cells differentiating neuronal cells β III tubulin marker was expressed in neuronal cells. The toxic effects of 1μg/ml Silibinin on stem cells were also demonstrated and it stopped the cell growth at the end of the first week. The maximum differentiation on stem cells in 0.5μg/ml Silibinin was observed to be significant (

One of the most important issues in promoting mother and child health is reducing the morbidity rate after cesarean section. The aim of this study was to investigate the influence of closure and non-closure of the visceral and parietal peritoneum on post cesarean morbidity in women attending Shabihkhani Maternity Hospital in Kashan Iran. This study was conducted with a single blind randomized clinical trial method on 100 parturient women that underwent emergency or elective cesarean section. Patients with previous cesarean section and or abdominal surgery diseases such as hypertension diabetes mellitus and premature rupture of membrane and pre operative bleeding were excluded from this study. Then the participants were randomly divided into two groups: in one group both peritoneal layers were closed while in the other group they were not closed. Post operative morbidity including fever bleeding post operative pain analgesic consumption and time of operation were assessed. Data were analyzed with t-tests and χ2 and a P.

Since occupational hazards are prevalent in emergency wards’ personnel due to occupational stresses and nonstandard work facilities and equipment and any negligence as to these personnel may reduce the quality of nosocomial services to improve the quality of the hygienic and therapeutic services provided by this ward the physical status of emergency wards’ personnel including physicians nurses workers and administrative personnel should be assessed as the first step. For this purpose it was the goal of this project to assess the physical status of the personnel of the emergency wards of hospitals affiliated to Qazvin University of Medical Sciences in Qazvin Iran and discover the relation it had with muscular-skeletal disorders. This study was a deh1ive-analytical one. The participants included all personnel of the emergency wards of Shahid Rajaei Hospital and Bouali Hospital and were selected from the four educational hospitals affiliated to Qazvin University of Medical Sciences. The occupational-bodily status of the subjects was first assessed by Rapid Entire Body Assessment Method. To study the muscular-skeletal disorders Nordic’s Standard Questionnaire was used. Finally the obtained data were analyzed by SPSS/win 16 software and statistical tests including Chi-Square and Coefficient Correlation Tests. In this study the maximum amount of prevalent pain was found to be 51% occupational accidents (6.7%) and the longest period of pain was found to exist in the waist area. In most cases “pain in the neck” was the factor limiting the work activities and it was also the reason for most cases of absenteeism and referring to physician during the last year. The body status score of most personnel was in the range of 4 to 7 according to REBA method. The risk proved to be at an average level and a corrective action in this regard seemed to be essential (71%). Ultimately the results revealed that no significant relation existed between REBA score of each member of the body and the pain felt in the same body part (P>0.05). The total REBA scores of each individual proved to have a significant reverse relation with the education level while it had a significant direct relation with stress intensity (P

One of the metabolic responses to surgical stress indicates itself in serum glucose level changes. Some studies have shown that hypoglycemia occurs after spinal anesthesia. In this study pregnant women’s blood glucose changes were measured before during and after cesarean section under Lidocaine spinal anesthesia. The study was conducted as a pre-post clinical trial. Full term pregnant women candidate for cesarean section having referred to Rasol Akarm Hospital and during 2007-2008 in Tehran were enrolled. All women underwent similar spinal anesthesia with Lidocaine 5%. Their blood glucose levels were measured simultaneously with glucometer on three occasions (before 0.5 hr after the beginning of anesthesia and during recovery). One hundred fifty four women enrolled in the study. The means of serum glucose level reduced after cesarean section. Although the mean of serum glucose level increased in recovery room it was significantly less than the baseline level (P.

Menopause can be associated with psycho-somatic changes in oro-facial areas like xerostomia and Burning Mouth Syndrome although these findings are controversial. The present study sought to compare the Un-stimulated Whole Saliva (UWS) flow rate of a group including menopausal & postmenopausal women and same-aged men. In this cross-sectional analytic-deh1ive study 40 menopausal & post-menopausal women (as experimental-group) and 40 same-aged men (as control group) without any systemic diseases and any drug consumption were divided into 2 groups xerostomia was evaluated by a questionnaire and their psychological conditions were assessed with HAD scale. UWS flow rate was measured by the spitting method. Data were analyzed by chi-square Krusscal Walis and Mann-Whitney tests. Mean of UWS flow rates in experimental group was significantly less than that in control group (P=0.006); no significant difference was found between the two groups regarding psychological condition. Also menopausal women had significantly greater xerostomia than men (45% vs 15%) (P=0.003). Based on this study xerostomia and reduction in UWS flow rate are sequences of menopause these findings necessitate the increasing awareness of menopausal & postmenopausal women for controlling the methods of these problems. Keywords: Menopause; Xerostomia; Saliva. Corresponding Author: Faculty of Medicine Yazd University of Medical Sciences

A Survey of Mother BMI Relationship with Cesarean Prevalence Rate in Bam Maternity Hospital Bam Iran Mahdyeh Solimanizadeh L. (MSc)1; Solimanizadeh F. (BS)2; Askaryzadehmahani M. (MSc)3; Zafarnia N. (MSc)3; Javadi M. (PhD)4 1Instructor of Nursing Bam Faculty of Nursing Kerman University of Medical Sciences Kerman Iran. 2Bachelor of Sciences in Nursing Ministry of Health Tehran Iran. 3Master of Sciences in Nursing Bam Faculty of Nursing Kerman University of Medical Sciences Kerman Iran. 4PhD Student of Nursing Yazd University of Medical Sciences Yazd Iran. Abstract Abnormality of maternal weight has an important role in reproductive health. High and low BMI play importaxt roles in the development of some pregnancy complications high birth weight and cesarean rate increas. Thus the complications such as sever infection bleeding thromboembolism attacks respiratory pneumonia in pregnant women undergoing cesarean occur more often. The aim of prenatal care is safety in infant birth and one of the conditions in pregnancy health care is normal weight. The present study sought to determine the role of mother BMI on cesarean prevalence rate in women referring to Bam Mahdieh Maternity Hospital in Bam Iran in 2005. This attempt was a comparative cross-sectional study done on 294 pregnant women who were divided into three groups: underweight n=111 (BMI

Eosinophilic gastroenteritis is a heterogeneous disorder affecting both children and adults and is characterized by the presence of an intense eosinophilic infiltrate on histopathology of one or multiple segments from the esophagus to the rectum. Eosinophilic gastroenteritis which may involve more than one layer of the gastrointestinal tract. Clinical features depend on the layer and location to be involved. Involvement of the serosal layer occurs in 10% of cases of eosinophilic gastroenteritis and is typically presented as ascites. The patient in our report was as likely as those who refer with full thickness involvement and presenting ascite and was completely improved after steroid treatment. Case Report The patient was presented a 14-year-old boy that referred to the physician with progressive abdominal pain and ascites. َAfter recognition of eosinophilia in blood and ascite fluid he was suspected to have Eosinophilic gastroenteritis. Ultimately following serial examinations and after laparascopic full thickness biopsy Eosinophilic gastroenteritis was closely diagnosed. The patient was treated with prednisolone. The abdominal pain and ascites subsided soon after initiation of steroid treatment. Keywords: Gastroenteritis; Ascites; Prednisolone.