مجله پزشکی دانشگاه علوم پزشکی تبریز
سال چهلم شماره 1 (پیاپی 133، فروردین و اردیبهشت 1397)

The aim of this research was to investigate the effect of four weeks aerobic exercise with moderate intensity on hs-CRP, IL-10, and BDNF in women with syndrome metabolic with the age of 50-65 years old.
24 women with metabolic syndrome (MetS) took part voluntarily and divided in two-group MetS exercise (ME), MetS control (MC). ME group participated in an aerobic exercise training (AT) program (4 weeks), three session per week, each session contained three performing parts and two rest part. Also, blood samples were conducted before and after training for evaluating levels of hs-CRP, IL-10, BDNF. Data were analyzed using Pried-sample T-Test, and Independent samples T-Test.
IL-10, BDNF after four-weeks aerobic exercise significantly increased (P˂0.05) also hs-CRP levels had no significantly differences. Hs-CRP, IL-10, BDNF after three months in control group did not significantly changed (P˃0.05).
These findings indicate four-weeks aerobic exercises induce to increase IL-10 level and did not change hs-CRP level. And BDNF's may increase relative to IL-10 level.

Despite advances in clinical and laboratory techniques, including stimulating ovulation and transferring embryo, success rate of pregnancy remains is about 30%. The aim of this study was to assess the effect of intra uterine G-CSF on implantation and clinical pregnancy rate in patients with implantation failure in IVF.
This study was a randomized clinical trial on patients undergoing in vitro fertilization within an age range of 45-18 years and a history of failure in implantation for two or more times. A total of 34 patients receiving G-CSF in the intervention group and 38 patients in the control group were enrolled.
Mean age of the participants was 34.1 years with a standard deviation of 6.4 years. The average number of years of fertility was 10 years with a standard deviation of 5.2 years. The average number of embryos implanted in the intervention group was 2.88 with a standard deviation of 0.33. The figures for the control group were 2.97 and 0.37, respectively. The two groups did not differ significantly. With respect to the potential clinical and laboratory fertility outcomes, including existing gestational sac with observed heart rate and positive serum βHCG, no statistically significant difference was observed between the two groups.
It seems that the effect of G-CSF on improving fertility outcomes in patients with implantation failure does not exist and more related studies are recommended in this regard.

Diazinon is an organophosphate insecticide that normally used to control different types of harmful insects in agriculture.
25 laboratory mature female mice divided into 5 equal groups: The control group did not receive diazinon, Sham groups of A and B, respectively, received0.52 and 5.2microliter of emulsifier, Diazinon experimental groups of A and B also received, respectively, 1.3 and 13 microliter form of inhalant.
In all histomorphometry and appearance studies, there was no significant difference between the control group and two sham groups but in the experimental group of B there were abnormalities such as atrophy of the fetus and placenta, cutaneous bleeding, the position of fetus was absorbed with extra placenta. The examination of intestinal Histomorphometry of embryos in the average number of goblet cells in a level equal to length of the villi in the experimental group of B in compared to the experimental group of A and the control showed that this increases were not significant. The average number of epithelial cells in a certain level of transverse sections of villi in both the experimental groups compared to control group showed no significant difference. Percentage of goblet cells in the entire villi’s in the experimental group A and B showed a significant decrease in compared to the control group.
Consumption of high levels of Diazinon in pregnant mice caused growth and development disorders and physical anomalies in the fetus as well as abnormalities in the development of intestinal tissue of embryos.

Poisoning with tetra carbon chloride produces free radicals in the body tissues including testes. In this research the protective effect of Avicennia marina leaf extract on the rat's spermatogenesis and the serum level of testosterone and LH&FSH induced with carbon tetra chloride are studied.
In this experimental study, 42 rats were divided into six groups including control group(receiving saline normal), sham( receiving olive oil), group induced by CCl4 ( receiving 2ml/kg carbon tetra chloride with 1:1 percent and olive oil featured as single dose), and experiment groups 1, 2, and 3(receiving 2ml/kg carbon tetra chloride with 1:1 percent and olive oil featured as single dose and the extract with daily dosages of 200mg/kg, 400mg/kg, and 800mg/kg). Two hours after injecting carbon tetra chloride, the extract was injected into peritonea once every 24 hours for 96 hours.
The normal and disable sperms in the group induced by CCl4 shows a significant reduction (P Avicennia marina leaf extract has protective effect against toxic effects of carbon tetra chloride because it contains anti-oxidants.

Helicobacter pylori are known as the most common species affecting human. This bacterium is main cause of peptic and duodenal ulcer. This infection is highly prevalent in the third world countries like Iran. This bacterium is found in dental plaque, salivary, adenoid and tonsil tissue, nasal polyps, nasal mucus, and maxillary sinus. These organs are near to the eustachian tube and middle ear, for this reason it is possible to find this bacteria in middle ear. PCR method is used for amplifying a particular piece of target DNA which can be a single gene or pieces of a gene.
This descriptive-analytical methods study was performed on 40 children between 2-13 years old with serous otitis media hospitalized in Tabriz Children Hospital (2007-2010). Serous fluids were collected from paracentesis of middle ear of patients was investigated for the PCR.
The patients were at the age of 2-13 years old and the average age was 6.57 years old. 21 patients (52.5%) were male and 19 patients (47.5%) were female. From 40 children with otitis media, helicobacter pylori were found in 3 children who showed no causality between helicobacter pylori and middle ear.
We can only assume a possible role for Helicobacter pylori infection at occurrence of middle ear infection. If this role is proved in the serous otitis resistant to common cures, antibiotics effective against H.pylori can be helpful.

Alopecia areata is an autoimmune disease, as a non-scarring hair loss and is without clinical signs. Although spontaneous recovery occurs in many patients with alopecia areata, but a lot of patients suffer from chronic periods of the disease. And according to the related serious complications of corticosteroid treatment and high relapse rate, finding alternative effective treatment for these patients by dermatologists is still ongoing.
50 patients with alopecia areata scalp during the last 3 months, who had no response to treatment with corticosteroids, were enrolled in two equal groups. One group received therapy with topical Diphenylcyclopropenone (DPCP) and the other group was treated with topical Anthralin. (IRCT: 201410113566N5).
Patients in the two groups in terms of gender, age, extent and duration of the current period scalp disease were not significantly different. No significant difference was observed in treatment response between the two groups (P=0.189). Age, as the only prognostic factor in treatment DPCP was found (P=0.047) and any prognostic factor in Anthralin group not found. More complications was seen in Anthralin group compared to the DPCP group (P=0.018). The duration of treatment and relapse rate, there was no significant difference between the two treatment groups.
Even better effect using DHCP in alopecia areata, but no significant difference Anthralin. But according to fewer side effects in DPCP and better effect on younger ages, this drug can be used as alternative treatment for resistant alopecia areata of children.

Obesity is one of the most important health problems in many countries which increase the incidence of cardiovascular diseases. Although cardiovascular findings are specific to adulthood, but identification of cardiovascular risk factors in childhood is necessary to prevent damage to target organs in adulthood. The aim of this study was to evaluate the frequency of cardiovascular risk factors in obese children and adolescents.
This cross-sectional descriptive study was performed on 442 children and adolescents aged 4 to 16 years old. After the clinical examination, blood pressure, height and weight were measured. The body mass index over than 95th percentile for age and sex or (BMI Z-Score equal or more than 2) was defined as obesity. After 12 hours of fasting the blood samples were taken for measurement of serum lipid profiles, insulin and glucose levels.
Of the total obese subjects, 15.8% (n=70) had no risk factors for cardiovascular disease, while 22.2% had one, 32.4% had two, 22.4% had three, 2.5% had four and 1.6% had five risk factors. The most common risk factor was hypertriglyceridemia (52.3 %) and the second risk factor was impaired fasting blood glucose (34.9%).
The prevalence of cardiovascular risk factors is high in obese children and adolescents.

Vascular endothelial growth factor (VEGF) plays an important role in development of new blood vessel and angiogenesis. Human VEGF121 is smallest member of VEGF family. Production of active and correct form of VEGF is the most challenging issue.
Here we described a method for optimization of refolding of VEGF121 which was expressed in bacterial host. Enzyme linked immunosorbent assay and proliferation assay of human endothelial cells was performed to monitor refolding and functional assay of VEGF121.
Using described method; VEGF was in correct fold and detected by antibody in ELISA. Furthermore, VEGF stimulated proliferation of human endothelial cells in dose-dependent manner.
Refolded VEGF has potential for stem cell differentiation.

Poor posture has been implicated in the development and perpetuation of chronic neck pain symptoms. The aim of this study was to evaluate the effects of six weeks corrective exercise on forward head angle, pain and timing of superficial neck muscles activation during posterior-anterior perturbation in women with chronic neck pain.
26 women with chronic neck pain and neck movement control impairment was selected for this quasi experimental study via simple noprobable sampling and divided into two corrective exercise (n=13) and control (n=13) groups. Forward head angle, pain and disability and timing of neck superficial muscles activation measured by using the photogrammetry, neck disability index and surface electromyography respectively in pre-test and post-test. Dropping the weight equal to 10% of total body weight, performed using electrical magnet, followed by pulling of the trunk inducing perturbation was performed. Kolmogrove smirnove, paired and sample t tests were used for statistical data analysis (α≥0.05).
The significant decrement in electromyographic responses of neck superficial muscles of upper trapezius (right P=0.001; left P=0.014), cervical erector spinea (right P=0.010; left P=0.001), sternocleidomastoid (right P=0.040; left P=0.003), neck pain and disability (P=0.003) and forward head angle (P=0.001) were found after six weeks of corrective exercise.
The corrective exercise intervention was successful at decreasing forward head angle, pain and disability and timing of muscle activation in subjects with chronic neck pain. Considering the extremely large effect size of the corrective exercise group, we recommend that this program be used in the correction of subjects with chronic neck pain in future.

Wound infection is not uncommon after cesarean sections. Using a first-generation cephalosporin (cefazolin) has been recommended for antibiotic prophylaxis in patients, but according to few reports, extending the spectrum of antibiotic prophylaxis by adding azithromycin to the standard cephalosporin may lead to extra protection against postcesarean infections. The relevant available data are not enough to draw a solid conclusion. This study aimed to compare the effectiveness of conventional antibiotic prophylaxis using cefazolin with a combination of cefazolin and azithromycin in reducing wound infection.
In this randomized clinical trial, candidates of cesarean section were categorized in two groups; cases (n=85) who received intraoperative cefazolin plus postoperative oral azithromycin (500 gr) and controls (n=86) who received intraoperative cefazolin only. The incidence rate of surgical wound infection by 30 days postoperation was compared between the two groups.
Cesarean sections were emergency in 80% of cases and 89.5% of controls (p=0.08). Postcesarean incisional wound infection developed in 4.7% of controls, whereas no patient in the case group had such infection (p=0.12).
Compared to cefazolin alone, a combination of azithromycin and cefazolin was more effective in preventing postcesarean incisional wound infection, the difference was not significant. Nonetheless, owing to this advantageous clinical consequence using a combination of these two antibiotics instead of cefazolin alone is recommended.

Curcumin has anti-inflammatory and antinociceptive effects and also it can be exploited for drug abuse treatment, but its low bioavailability and water solubility are the most important obstacles and limit its application. The recent achievements of nanotechnology illustrate a novel approach in treatment of drug abuse and addiction. The present study was conducted to investigate the effect of dendrosomal Nano curcumin (DNC) administration on morphine withdrawal signs in rats.
Male rats (250-300 gr) were anaesthetized and implanted with silastic catheters inserted into the right jugular vein. Then they were placed in the self-administration apparatus for 2 hours/day for 12 days and received saline or morphine (5mg/ml) by infusion pump. In addition, DNC (10, 5 mg/kg.IP) were injected 30 minutes before placing in apparatus and the signs of withdrawal were measured for 30 minutes after injection of naloxone (2 mg/kg, i.p).
Morphine significantly induced dependence and withdrawal signs in sham group. On the other hand injection of DNC significantly decreases these signs (p DNC can decrease withdrawal signs of morphine and can be used as an effective pharmacotherapy for morphine abuse.

Cancer is one of the most important factors which affect the human health. RNA interference (RNAi) based therapies have been developed and showed significant results in this regard. One of the most important problems of siRNA system is its low stability. In order to overcome to this weakness, various types of hairpin RNA or shRNA was introduced. Over expressed colorectal cancer gene (OCC-1), which has several transcripts is considered as a hallmark of colorectal cancer. This gene has vital role in the development and progression of cancer through the cell signaling pathways, which are involved in cell proliferation. In the present study, the RNAi system was utilized in order to reduce the expression of specific transcript of OCC-1 gene.
The silencer structure was designed and cloned in the expression vector pRNA-H1.1 / Neo which has a promoter for RNApolIII. Plasmid was transferred into the colorectal cancer cell line, and the expression of transcript was compared to the control samples at 24 and 72 h post transfection by Real-Time PCR.
The expression of transcript was reduced about 25 % at 72 h post transfection following shRNA transfection, compared to the control sample.
The designed shRNA structure successfully reduced the expression of target gene; accordingly, it can be used in future studies in order to investigate the effect of these transcripts on development and progression of colorectal cancer.

Plexiform angiomyxoid myofibroblastic tumor is a very rare mesenchymal tumor of stomach which is diagnosed based on morphologic findings such as plexiform growth pattern, spindle cell proliferation in a myxoid background, by aiding immunohistochemistry staining and ruling out of other mesenchymal gastric tumors. We report this tumor with about 4cm size in antrum of a 38 years old man which endoscopic and CT scan results fit with Gastrointestinal stromal tumor and diagnoses is performed according to specific morphologic and immunohistochemistry findings.