فهرست مطالب

فصلنامه آزمایشگاه و تشخیص
پیاپی 43 (بهار 1398)

تاریخ انتشار: 1398/03/30
تعداد عناوین: 20

فارسی
English

انتخاب همه

The role of miRNA in Recurrent Spontaneous Abortion

Fatemeh Karimi khouzani, Sadegh Valian Bourojeni* Pages 8-22

Abortion is a term used for missing of fetus before 20th week of pregnancy, which is a common complication of pregnancy and its aetiology usually remains unknown. Known aetiologies for this phenomenon include structural malformation of uterus, genetic anomalies, autoimmune conditions, endocrine defects, coagulative and trombophilic disorders, polycystic ovary, obesity, and lifestyle. Abortion has many mental and physical consequences for the involved couple, especially when it becomes recurrent. Analysis of molecular changes of microRNAs expression that are changed in abortion can help identifying people who are susceptible to abortion and even prevent it. MicroRNAs are a group of non-coding RNAs that regulate a large number of genes. This regulation can occur at transcription level or after transcription. Expression of microRNAs is associated with a variety of diseases. Studies have shown that during abortion, expression of some of these microRNAs is altered (both decrease and increase), which has a large effect on expression of their target genes. Single nucleotide polymorphism (SNP) in coding genes of microRNAs could affect microRNAs’ expression. In the present article, the role and effects of MIRs on recurrent abortion is reviewed.
Keywords: Recurrent abortion, microRNA, ERBB2 gene, LIF gene, MIR-125a, rs12976445

Abstract View Paper
Nail Diseases: Lichen planus-section 9

Mohammad Ghahri Pages 23-27

Lichen planus affects about 1%-2% of the general population. There is little known as to the cause of the condition, but all theories include a reaction of the body’s immune system. Lichen planus can develop nearly anywhere on the body. On the skin, lichen planus “appears as rows of itchy, flat -topped bumps,” It can also appear on the inside of the cheek and on the gums and tongue. Lichen planus also appears on the scalp (referred to as lichen planopilaris). On the scalp, lichen planopilaris can cause permanent damage to hair follicles and leave adults with patches of baldness. Of those affected with the condition, about 10% will have lichen planus on the nails. The condition can cause splitting or thinning of the nails. One reason is it’s easy to confuse with other nail conditions, such as pterygium. Another roadblock to a diagnosis is the fact that lichen planus can show up on all the nails and toes, on only the nails or the toes, or on only select nails or toes. However, when lichen planus appears on the nail, it often destroys the matrix. Consequently, even if the lichen planus clears up, the nail will still be damaged. Lichen planus can be itchy, and it can cause inflammation. It may not be possible to clinically differentiate nail involvement by lichen planus from involvement by psoriasis, primary onychomycosis or traumatic nail unit dystrophy. One could reach a diagnosis of lichen planus on the basis of the typical clinical appearance and a punch biopsy of a representative lesion.
Keywords: Nail diseases, Nail dystrophies, Nail disorders

Abstract View Paper
A Comparative Study of Managerial Intelligence among University Students: School of Allied Medicine of Tehran University of Medical Sciences in 2017-2018

Hossien Dargahi, Negin Bashari, Arash Akbarzadeh Pages 28-41

Introduction
In new management approach ,using managerial intelligence has special significance, that includes emotional, mathematical/logical, political, cultural, spiritual and moral intelligence. So, this study is aimed to compare managerial intelligence among students of school of Allied Medicine of Tehran University of Medical Sciences in 2017-2018.
Method
A descriptive-analytical study was induced using sample size by krejcie and morgan table was consisted 225 students selected based on random classification method. For collecting data, managerial intelligence questionnaire was used included demographic information and 6 managerial quotient elements. 206 questionnaires were collected and they were analysed with descriptive statistic and Kolmogorov-smirnov test, T-test, Wilcoxon-singled test and U Mann- Whitney test by SPSS 21.
Findings
The least managerial intelligence score of students was 372.1,and the highest score was705.5 and the average managerial intelligence score of students was 569.12±51.5,and the mean was more than its expected amount. There was no meaningful correlation between managerial intelligence of male and female students and also between different major fields. But, these findings indicated that MSc. and Ph.D students had higher scores than undergraduate students in managerial intelligence.
Conclusion
Regarding of high importance of managerial quotient for health care system employees´ performance after graduation and starting employment, it is necessary that managerial quotient should be selected as a most important criterias for selection of competent health care system managers.
Keywords: Managerial Intelligence of Students, School of Allied Medicine, Tehran University of Medical Sciences

Abstract View Paper
Evaluation of the Effects of Morphine on Sex Hormones in Wistar Rats

Mohammad Tashauoei, Amir Bahrami, Fariba Nabatchian* Pages 42-49

Background and Aim
Morphine is one of the most important alkaloids of opium family that is the most effective combintion of family as well. Morphine is a narcotic analgesic and is very strong. Due to the use of morphine and its derivatives, applying the effect of this substance on the body's hormones, especially sex hormones is very important. The aim of this study was to investigate the effect of morphine on sex hormones and the effect of morphine on gender differences.
Materials and Methods
The study, an Applied study in which 38 Wistar rats (wistar) was used. In the blood serum were measured 17 beta-estradiol, progesterone and testosterone using biochemical kits .
Discussion
Our study shows that testosterone levels within 1 week after taking morphine compared with the control group showed a significant decrease. This decrease morphine consumption continues until the fourth week. Our study shows that hormone levels in the first and second weeks, the first and third difference is not significant. The progesterone in between the second week than the first week of morphine showed a significant reduction (002/0 P <) in mice, according to sex, but the comparison between the second and first weeks was not significant
There was no relationship between morphine and estradiol levels.
Conclusion
The results show a significant decrease in testosterone with morphine. This reduction would be associated with decreased libido and male fertility. The hormone progesterone indicate that this reduction could be significantly reduced morphine consumption and maintenance status of the embryo at this stage of pregnancy related.
Keywords: : Morphine, Testosterone, Progesterone, Estradiol

Abstract View Paper
Investigating of the methods used to find external agents in vaccines,biological products

Majid Jamshidian Mojaver*, Hamidreza Farzin Pages 50-54

multiple technological levels the necessity of developing vaccine quality control systems is one of the requirements of our country Coordinated tests of the absence of externous agents in biological substances are considered as a major international concern for veterinary vaccines. Exogenous agents such as Newcastle disease virus, avian leukosis virus, infectious laryngotracheitis virus (ILTV), infectious bursal disease virus, infectious bronchitis virus, chicken anemia virus, hemorrhagic enteritis virus and reticuloendothelial virus. Identification methods for these external viral agents include: Serological testing of virus specific antibodies in vaccinated birds, virus isolation in vaccinated birds, virus isolation in embryonic eggs or cell cultures. Here's an overview of these methods.
Keywords: quality control, biological substances, externous agents

Abstract View Paper
Laboratory diagnostic methods of malaria infection

Reza Bohloli Khiavi Pages 55-59

Malaria is one of the most important tropical infectious diseases. The incidence of malaria worldwide is estimated to be 300–500 million clinical cases each year with a mortality of between one and three million people worldwide annually. The accurate and timely diagnosis of malaria infection is essential if severe complications and mortality are to be reduced by early specific antimalarial treatment. This review details the methods for the laboratory diagnosis of malaria infection.
Keywords: malaria, diagnosis, microscopy, rapid

Abstract View Paper