Zahedan Journal of Research in Medical Sciences
Volume:18 Issue: 7, Jul 2016

High blood pressure (BP) is considered as a strong predisposing factor for cardiovascular diseases (CVD) Environmental and genetic factors may have a role in high blood pressures. Nutrition has a potential role in the prevention of hypertension and its sequelae. Effect of lowering blood pressure by modification of complex dietary patterns may be the result of synergism between the various components of certain foods or food combinations rather than of the specific effect of a particular nutrient. Vegetarian Diet, Mediterranean Diet, and Dash Diet are three food patterns which have been associated with lowering BP. Vegetarian Diet are characterized by high intake of legumes, nuts, vegetables, and fruits. A relatively high polyunsaturated to saturated fat ratio will make by this kind of diet. Low fat content and high potassium, magnesium, and fiber content of this diet, all factors possibly cooperating to the reduction of blood pressure. Mediterranean Diet has low animal and dairy products as well as saturated fatty acids and cholesterol; it is rich in plant food, legumes, fiber, and antioxidant vitamins with olive oil as the main source of fat. The dietary approaches to stop hypertension (DASH) is a success process in control of hypertension, which emphasizes vegetables and fruits and dairy foods with low-fat, it also includes more nuts, poultry, fish, and, whole grains and lower amounts of red meat, fats, sweets, and sugar-containing beverages. DASH diet is poorer in total and saturated fat and cholesterol and richer in potassium, magnesium, calcium, and fiber in comparison to the habitual Western diet. In conclusion, although multiple dietary factors can influence on BP and each factor has a modest effect; the combined effects of those factors can be substantial. In the current study, we review food patterns, lifestyle, and their relationship with hypertension and the possible mechanisms involved.

Ischemic stroke is one of the most frequent reasons of adult disability. Newly, few trials have represented that fluoxetine might meliorate functional recovery after stroke.
Our purpose of this study was the evaluation of effectiveness of fluoxetine in rehabilitation of patients after stroke.
In this double-blind trial study, patients who admitted for stroke, participated in a double-blind, randomized, clinical trial study with fluoxetine (20 mg once per day, orally), and placebo. They were examined for alterations in a depressive mood, evaluated by the Hamilton depression rating scale (HDRS) and changes in motor impairment, assessed by the Barthel index (BI). assessments of patients were done in days 0, 45 and 90 with BI and HDRS scales according to their functional treatment response. Statistical analyses were performed using the SPSS-16 software.
One hundred seventy two patients were randomly assigned to fluoxetine (n = 86) or placebo (n = 86), and 150 were included in the analysis (75 in the fluoxetine group and 75 in the placebo group). BI improvement at day 90 was significantly greater in fluoxetine group (3.09 ± 1.57 points, P Early prescription of fluoxetine has significant effect on motor improvement after ischemic stroke.

Human leukemia inhibitory factor (hLIF) is a poly functional cytokine with numerous regulatory effects on different cells. Main application of hLIF is maintaining pluripotency of embryonic stem cells. hLIF indicated effective work in implantation rate of fertilized eggs and multiple sclerosis (MS) treatment. Low production of hLIF in eukaryotic cells and prokaryotic host’s problems for human protein production convinced us to develop a simple way to reach high amount of this widely used clinical and research factor.
In this study we want to purify recombinant human leukemia inhibitory factor in single simple method.
This is an experimental study, gene expression: human LIF gene was codon optimized for expression in Escherichia coli and attached his-tag tail to make it extractable. After construction and transformation of vector to E. coli, isopropyl β-D-1-thiogalactopyranoside (IPTG) used for induction. Single step immobilized metal affinity chromatography (IMAC) used for purification confirmed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and western blotting. Bioactivity of the hLIF were tested by MTT assay with TF-1 cells and CISH gene stimulation in monocyte and TF-1 by real-time PCR. Induction by 0.4 mM of IPTG in 25°C for 3 hours indicated best result for soluble expression. SPSS indicated P 0.05 that is significant for our work.
Cloning, expression, and extraction of bio active rhLIF was successfully achieved according MTT assay and real time PCR after treatment of TF-1 and monocyte cell lines.
We developed an effective single step purification method to produce bioactive recombinant hLIF in E. coli. For the first time we used CISH gene stimulating for bioactivity test for qualifying of recombinant hLIF for application.

Alzheimer’s disease is one form of dementia in old age. Alzheimer’s disease is among the incurable diseases which usually show its symptoms in the seventh decade of human life. The disease may be present in the body for years without the incidence of the symptoms. Considering many research studies on this disease, the cause of Alzheimer’s disease is still unknown. Alzheimer’s disease occurs hereditary or accidentally in old people.
Based on the importance of Alzheimer’s disease and lack of a cure (definitive treatment) for this disease, we decided to use Data mining method to identify the hidden patterns in effective genes causing the incidence of this disease.
In this analytical study, we identified the genes with altered expressions in patients diagnosed with Alzheimer’s disease. In this research, we identified genes with an increase and decrease in gene expression in patients diagnosed with Alzheimer’s disease and then presented the important sequences found in each gene of this disease as Alzheimer’s biomarkers. Microarray libraries related to Alzheimer’s disease were used and finally, we weighted the data using 10 data mining methods.
The sequences gain a weight more than 0.5 in at least two of the weighting algorithms, would be chosen as the most important sequenses.our work shows that TGCCCC; AGCCTG; GAATAT, AATTG; And AAATTG are the most important sequences since these sequences have been chosen as the most important sequences in 8 repetition of weithening algorithms
The results of this thesis are consistent with the results obtained from the previous researches and confirm the previous assumptions. The important effect of beta amyloid and E- T PAZ and Kinase and microglobulin genes in Alzheimer’s disease is validated in the previous researches too.

Curcumin is the natural yellow pigment in turmeric isolated from the rhizome of the plant Curcuma longa. Curcumin inhibits formation and invasive cancer cells and destroys cancer cells resistant to chemotherapeutic drugs.
The purpose of this study was the survey of effects of different concentrations of alcoholic curcumin on the octamer-binding transcription factor 4 (OCT4) Nanog and Nucleostemin genes in the AGS (human gastric adenocarcinoma) cell line.
In this experimental study the AGS cell line was cultured in RPMI-1640, supplemented with penicillin/streptomycin (100 U/mL and 100 mg/mL, respectively) and 10% fetal bovine serum, at 37°C in a humidified atmosphere of 5% CO2. In 60 - 70% cell confluence, the cells were treated with curcumin concentration (20, 40, 100) μL and incubated for 24, 48 and 72 hours. Finally, total RNA were extracted and cDNA were synthesized and the expression of mentioned genes was detected. The data were analyzed by excel software.
Expression rate of OCT4A, OCT4B, Nanog and Nucleostemin (GLN3) at concentrations less than 20 μg/mL were reduced but OCT4B1 expression showed increased by hours respectively.
The results showed that curcumin inhibited cell division; also, this study could be the basis for more extensive studies on the anti-cancer effect of the combined plants.

Diagnosis of acute appendicitis in pregnancy is difficult. Delay operation, increase complications.
This study focused on early operation on base of careful history, precise physical examination, and rational close observation and evaluates its results with conventional investigation in pregnant women suspected acute appendicitis.
A cross sectional study in100 pregnant women and 100 aged matched non pregnant women underwent appendectomy during Sep 2011 - Dec 2014. The data were analyzed by chi-square test through SPSS 16.0.
Age 16 - 37 years, mean age in pregnant women and no pregnant women were24.75 ± 4.4 and 27.56 ± 6.53 years (P > 0.05), respectively. 20 - 25 years age group, were more frequent = 44%. 70% patients were gravid 1, mean hospital stay in pregnant women, and non-pregnant women were 48 ± 6 and 85.2 ± 43.19 hours (P value There are no diagnostic laboratory findings in acute appendicitis during pregnancy. Careful history and physical examination and close observation of 12 ± 8 hours are sufficient for surgery decision making. In spite of high negative appendectomy since it has no Surgical and obstetric complication, early Appendectomy without aggressive investigation recommend.

The freezing of immature testis tissue and then the transplant of it can be considered as a major step in fertility preservation for young boys with cancer.
The objective of this study was to investigate antioxidant effects of vitamins E and C on testis tissue structure in the two types of freezing-thawing (fast and slow).
In this experimental study, 16 immature male mice (6 to 8 day-old) surgery, and testes were removed. The testes were divided randomly into 4 groups: control group and three experimental groups including: vitamin E, vitamins C and vitamins E and C. Testes placed in 4 prepared freezing solutions. These solutions, which were: 0.1 mM solution of vitamin E, 1 mM solution of vitamin C, solution of the vitamins E and C with mentioned doses and control solution, were prepared in the DMEM (Dulbecco’s modified eagle medium) base culture. Of each group were 4 testes into fast freezing-thawing procedure and 4 testes were into slow freezing-thawing procedure. Finally these testes, for analyzing injury, were stained with hematoxylin-eosin.
In the fast freezing-thawing procedure, seminiferous tubules injury significantly reduced in any of the three experimental groups compared to the control group (P = 0.01). At the slow freezing-thawing procedure, seminiferous tubules injury any of the three experimental groups significantly reduced compared to the control group (P = 0.01). In both procedures, there was no significant difference between the three experimental groups.
It seems that vitamins E and C due to its antioxidant properties protects seminiferous tubules from oxidative injury.

In surgical treatment of thoracolumbar burst fractures, most authors try to lower the number of vertebrae involved during the surgery.
The aim of this study was to evaluate the outcome of a medium-segment posterior spinal fixation in these patients.
Patients and We retrospectively reviewed 27 patients (18 male, 9 female) with mean age of 39.4 ± 15.0 years old in a before-and-after study. The mean follow-up period was 38.4 ± 15.6 months. We involved 2 intact above vertebrae and one intact below vertebra, inserting a pedicular screw at the fractured level and supplemented the construct with contralateral infralaminar hook. Clinical and radiologic characteristics were assessed with American spinal injury association (ASIA) scale, oswestry disability index (ODI), visual analogue scale (VAS), and plain radiography. Data analysis was carried out by SPSS version 11.5 software.
Mean post traumatic kyphosis was 15.7° ± 3.3° that was changed to - 8.5° ± 4.3° and ° ± 4.4° at immediate and last visit after surgery, respectively. Mean loss of correction (LOC) was 9.5° ± 1.9° (P In surgical treatment of thoracolumbar burst fractures, a medium-segment posterior spinal fixation, although cannot maintain the radiologic reduction of the fractured vertebrae efficiently, is not only associated with acceptable clinical outcome but also spare one lower intact lumbar segment and therefore recommended.

Attention deficit and hyperactivity disorder (ADHD) has a profound impact on lives of thousands of children and their families.
Aim of this study was to determine effectiveness of training for mothers, on these children.
In this quasi-experimental research, 30 mothers were randomly divided into control and experimental groups and the experimental group received Barkley management skills training in 9 sessions.
Findings showed that anxiety in children of trained mothers was decreased and self-esteem was increased.
The training program for mothers can be an effective method for treatment of psychological disorders in children with ADHD.

Extended-spectrum beta-lactamases (ESBLs) represents a significant and rapid emerging problem around the world. Nowadays, antibiotic resistance determinants are prevalent in both hospital and community strains of Gram-negative enteric bacteria.
In this sectional-descriptive study, 87 clinical isolates of Klebsiella pneumoniae were collected from outpatients and their susceptibility to antimicrobial agents, minimum inhibitory concentration (MIC) values for third-generation cephalosporins and ESBLs production were assessed.
Of 87 K. pneumoniae clinical isolates, 20 (22.9%) isolates represented the ESBLs phenotype.
Our results demonstrated that the resistance to the third-generation cephalosporins in K. pneumoniae strains isolated from outpatients is relatively high in Zahedan.