فهرست مطالب
Zahedan Journal of Research in Medical Sciences
Volume:18 Issue: 8, Aug 2016
- تاریخ انتشار: 1395/06/20
- تعداد عناوین: 10
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Page 1BackgroundIVM (in vitro maturation) appear to be certified high pregnancy outcome. Therefore, attempt to find an appropriate culture system using natural products for increasing developmental competencies fascinating.ObjectivesThis experiment is aimed to evaluate the effect of sea cucumber methanol extract on in vitro maturation of immature mouse oocyte.MethodsIn this experimental study, Preantral follicles at germinal vesicle stage were collected from 24 - 26 days NMRI female mouse ovaries and transferred to IVM medium supplemented with different concentration of sea cucumber methanol extract (5, 10, 20, 30 µg/mL). Antioxidant capacity of sea cucumber extract was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. Further, oocyte maturation and TNF-α expression were recorded till day 10th. Data were analyzed by one-way analysis of variance (ANOVA) through SPSS 16.ResultsThe percentage of arrested oocyte at germinal vesicle stage in the control group was significantly (PConclusionsIt was concluded that the sea cucumber extract with optimum concentration can improve oocyte maturation. Sea cucumber extract treatment can be suggested as a novel therapeutic strategy to be used in infertility modality in the future.Keywords: Oocyte, In Vitro Maturation, Antioxidant, Sea Cucumber
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Page 2BackgroundTwo popular indicators for detecting obesity are body mass index (BMI) and waist-to-hip ratio (WHR).ObjectivesThe primary purpose of this study was to examine the agreement between the BMI and WHR classifications in diagnosing obesity among adult Iranians by using cutoff points of world health organization (WHO) gold standards. The secondary purpose was to estimate suitable cutoff points for BMI and WHR for various sex and age-groups.MethodsThe study was cross-sectional. The data were obtained from 13,691 females and 5,675 males between 2009 and 2014. Body composition indices were measured by bioelectrical impedance method (BIA). Percentage of body fat (PBF), total fat (TF), BMI, and WHR were determined. Data analysis included t-test, chi-square test, receiver-operating characteristic (ROC) curves, Fishers r-to-z transformation, linear regression, and Hanely formula.ResultsIn the WHR classification, nearly 78% of obese subjects had been wrongly classified as non-obese, but only 15.8% of obese females were wrongly classified as non-obese. The WHR was ineffective in detecting obesity among males between 20 and 39 years old. By the BMI classification, 42.5% of obese males had been wrongly classified as non-obese, but it was effective in detecting obesity in females between the ages of 20 and 39 years.ConclusionsThe suitable BMI cutoff points for detecting obesity for adult Iranians are 27.7 in all subjects, 27.7 in females, and 27.3 in males, which are all less than the BMI of 30 recommended by the WHO. On the basis of the WHR, they are 0.88 and 0.83 for males and females, respectively, which are less than the recommended cutoff points for Europeans but similar to those Eastern Asians.Keywords: Body Mass Index, Obesity, Waist, to, Hip Ratio
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Page 3BackgroundToxoplasma gondii is an obligate intracellular protozoan parasite which has significant medical and veterinary impact on all around the world. Tracking of specific antigens or antibodies for toxoplasmosis is the main choice in its diagnosis. Recombinant proteins will improve sensitivity and specificity and reduce problems of standardization and reproducibility of diagnostic kits. Toxoplasma gondii Subtilisin-like protein (TgSUB1) is a novel example of a glycosylphosphatidylinositol) GPI (-anchored protein which can be considered as a potential marker for serodiagnosis of toxoplasmosis.ObjectivesThe aims of this study were to find out major antigenic parts of this whole protein and to develop a recombinant prokaryotic plasmid.MethodsIn this experimental study, using bioinformatics softwares Parker Hydrophilicity prediction and Bepipred linear Epitope prediction to select best highly antigenic region of this protein, a 744 bp fragment was amplified by polymerase chain reaction (PCR) on cDNA obtained from T. gondii RNA. The PCR product was cloned in PCR2.1 vector and subcloned into expression pET28a vector. The PCR2.1-SUB1 and PET28a-SUB1constructs were analyzed by PCR, restriction analysis and sequencing.ResultsA highly antigenic region in the hydrophilic part of the protein including amino acid residues 549 to 795 was successfully cloned and the sequences were confirmed. All nucleotide sequences in the PCR product have 100% homology with the published reference sequence.ConclusionsPairing bioinformatics tools and cloning of the candidate molecules in vaccine development studies and diagnostic approaches will have powerful impact on promotion of research in infectious diseases. This strategy is considered as available and inexpensive technology even in less developed countries where the infectious diseases like toxoplasmosis is prevalent.Keywords: Cloning, Subtilisin, Like 1, Toxoplasma gondii
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Page 4BackgroundFew studies have been done on free water excretion capacity in renal transplant recipients.ObjectivesThis study was designed to compare electrolyte free water clearance (E-CH2O) in renal transplant recipients with healthy control group.MethodsA cross-sectional study was conducted to determine three-hour creatinine clearance (CCl), E-CH2O, and percent urine output in 3 hours after administering 20 mL/Kg oral water loading following 12-hour fasting, in 22 renal transplant recipients with good graft function (creatinineResultsWe recruited 25 recipients (73% patients in living and 27% in cadaveric donor transplantation) and 26 healthy control groups.3 patients refused to collect urine and finally 22 patients finished the protocol. The mean age of subjects was 37.68 ± 13.88 and 31.40 ± 8.20 years old in renal transplant recipients and control groups, respectively. In patient group male to female ratio was 9/13 and in control group was14/12. Although the 3h-CCl was similar (126.49 ± 53.52 vs. 109.99 ± 47.06 mL/min) in two groups (P > 0.05), the E-CH2O (1.83 ± 1.22 vs. 2.94 ± 2.02) and 3-hour urine output percent (55.93 ± 22.86 vs74.11 ± 30.38) in kidney recipients was significantly lower than healthy controls (PConclusionsThis study demonstrated that E-CH2O has been decreased in renal transplant patients in comparison with control group when there was not any difference between3h- CCl in two groups. This indicates that E-CH2O may be decreased earlier than 3h-CCl in transplant patients.Keywords: Kidney Transplantation, Water, Electrolyte, Clearance
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Page 5BackgroundAlzheimers disease is a form of dementia in old age. Alzheimers disease is an incurable disease, usually shows its symptoms in the seventh decade of human life. This disease can exist in an individual for years without visible symptoms. The causes of this disease is still unknown although many studies have been done on this disease. Alzheimers disease appears in an old individual based on genetic and accidental factors.ObjectivesAccording to the importance of Alzheimers disease and lack of a definite treatment for this disease, we decided to identify the effecting genes on this disease by using Micro-array technology.MethodsIn this analytical study, Microarray technology was used to identify genes with over and down expression in patients diagnosed with Alzheimers disease and finally, we introduced up- and down-regulated genes by using Bayes T test.ResultsXIST gene, with 8.68652 over expression showed the highest level of over-expression while COL5A2 had 4.561804 down-expression which showed the most changes.ConclusionsFindings of this study, validate the previous assumptions and the only difference is in the expression level of genes which is normal due to differences in the sample.Keywords: Alzheimer's Disease, Micro, Array, Gene, Bayes T
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Page 6BackgroundFever is a natural response of the host to infection and a normal part of children''s infectious disease.ObjectivesThe purpose of the study was comparison of the combined treatment of acetaminophen and ibuprofen compact with each treatment alone.MethodsThis Double-blind clinical trial study was done on 540 children with 38°C to 41°C as body temperature. Eligible children after considering inclusion criteria divided in three groups randomly. First group of 183 patients administrated with acetaminophen, the second and the third groups of 178 and 179 patients with ibuprofen and combination. The first dose of antipyretic drug was administered to the patient under the supervision of a physician or nurse. After explanation of benefits and marginal effects to the parents if they accepted the conditions their children were admitted to the study. Parents were free if they wish to withdraw the study before completing. Information of each patient was recorded on a form. The data were analyzed by descriptive statistic, one-way ANOVA and SPSS software version 16.ResultsOut of sample 60.6% were boy. The mean age of children treated with acetaminophen, ibuprofen and combination therapy was 2.21 ± 2.49, 3.00 ± 2.92 and 2.22 ± 2.33 years in the order given. The results showed statistical difference in two (F = 4.45 and P = 0.012) and four hours (F = 3.11 and P = 0.045) after taking drug. A significant difference not observed in the value of temperature decrease within 2 - 4 hours after drug intake, (F = 2.49, P=0.084) but in the time of 0-2 (P = 0.012) and 4-6 hours (P = 0.001) was observed.ConclusionsThe findings of this study showed that acetaminophen is more effective for a short time but the combination in the long time when ibuprofen placed in the middle position with the respect of time.Keywords: Fever, Acetaminophen, Ibuprofen, Combination, Impact
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Page 7BackgroundThe nociceptin/orphanin FQ (N/OFQ) peptide is the endogenous ligand for the N/OFQ peptide (NOP) receptor. Beta adrenergic receptors and N/OFQ are involved in the regulation of food intake through central and peripheral mechanisms.ObjectivesThe aim of this study was to determine the possible interaction between beta adrenergic and N/OFQ receptors on food consumption in male rats. N/OFQ agonists have been shown to increase food intake in mammals and birds. Beta adrenergic receptors physiologically inhibit food intake in mammals.MethodsIn this experimental study were used male rats (250-300 g). In first two experiments, rats received intraperitoneal injections of isoproterenol (0, 0.5, 1 and 2 mg/kg) and Nociceptin agonist 64 - 6198 (0, 1, 2 and 4 mg/kg) respectively. In third experiment 64 - 6198 (4 mg/kg) and (2 mg/kg) isoproterenol were injected. In experiments 4 effects of isoproterenol (0.5 mg/kg) and Nociceptin antagonist (8 mg/kg) on food consumption was investigated. Latency time to feeding was examined in experiment 5. The data were statistically analyzed using One-way ANOVA and Tukey test.ResultsLatency time to feeding increased and decreased by isoproterenol and nociceptin/orphanin, respectively. Administration of N/OFQ increased cumulative food intake in rats (PConclusionsOn the basis of these findings, it can be concluded that isoproterenol may decrease food intake in rats by reduction in the N/OFQ content.Keywords: Food intake, Isoproterenol, Nociceptin, Orphanin FQ Rat
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Page 8BackgroundRefractive error is a common abnormality and the most common refractive error is myopia.ObjectivesThe aim of this study was To Comparison of rate of myopia among carpet weaver and non-weaver members.MethodsIn this case- control study, all members of the weavers have been refracted objectively and subjectively by only one person.ResultsThe relative frequency of moderate and severe myopia in carpet weaving significantly more than non-weavers (PConclusionsSustained close distance working could have an effective role in progression and amount of myopia.Keywords: Myopia, Carpet Weaver, Non, Weaver Members
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Page 9BackgroundIn iron deficiency anemia (IDA), serum ferritin level is decreased and total iron binding capacity (TIBC) is increasedObjectivesIn this study the relationship between H. pylori infection and iron deficiency anemia were studied.MethodsThis research was a cross-sectional study. TIBC and ferritin levels were measured in 133 patients, suspected to H. pylori infection, then serum levels of IgM and IgG antibody against H. pylori infection were measuredResultsFrom 133 patients, 97 were females (72.93%) and 36 males (28.07%), 36 of them (23 women and 13 men) have a TIBC levels more than 400 ug/dL and ferritin levels were less than 12ng/dL. Twenty patients had IgG antibodies and 5 patients had IgM against H. pylori.ConclusionsThe results show the association between H. pylori infection and increased levels of TIBC and decreased serum ferritin.Keywords: Ferritin, Total Iron binding Capacity, Iron Deficiency Anemia, Helicobacter pylori Infection
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Page 10BackgroundReproductive problems such as impaired folliculogenesis and anovulation are observed in diabetic women.ObjectivesIn this study the protective effects of Aloe vera (a traditional herb) on oocytes and ovarian folliculogenesis, were investigated.MethodsFor this purpose 25 mice were divided in five groups: control, Aloevera trated mice, insulin treated and aloe vera treated diabetics and diabetics. Diabetes were induced by a single injection of stereptozotocin (190 mg/kg, intraperitoneally). Ovulation and maturation rate, oocytes quality and ovarian folliculogenesis were studied after super ovulation.
Results andConclusionsThe results suggest that Aloevera has protective effects on the ovary and oocyte in STZ induced diabetic miceKeywords: STZ, Aloe Vera, Insulin, Ovulation, Folliculogenesis, Mice