مجله علوم پزشکی پارس
سال شانزدهم شماره 2 (پیاپی 44، تابستان 1397)

Incidence of drug resistance against chemical antimicrobial drugs has led the use of medicinal plants for treatment of infections in recent years. The aim of this study was to examine possible antibacterial and antifungal effects of Methanolic, Ethanolic and Aqueous extracts of Ocimum basilicum plant in vitro. After preparing the extracts with maceration method, antimicrobial activities and Minimum inhibitory concentration were examined by Disk-Diffusion, serial dilution method against bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and fungi Aspergillus niger and Candida albicans. To standardize of study, we used anti biogram disks and ATCC bacteria. The results showed that, the methanolic extract of Ocimum basilicum had maximum antimicrobial activity against Staphylococcus aureus and Aspergillus niger with the diameter of inhibition zone (20±1.02 and 18±1.20mm) respectively. In addition, the minimum inhibitory concentration of Methanolic extract against Staphylococcus aureus and Aspergillus niger was documented in 12.50 and 25 mg/mL, respectively. The aqueous extract of the Ocimum basilicum did not have a good effect compared to methanolic and ethanolic extracts. Discussion & conclusions: The Ocimum basilicum methanolic extract has significant inhibitory role in the growth of pathogenic bacteria in vitro. Meanwhile, more researches are recommended due to reaching more inclusive results.

The purpose of this study was to compare the effect of 8 weeks combined training and conjugated linoleic acids supplementationon on serum levels of resistin , Fasting Glucose,insulin resistance and Cardiovascular risk factors obese women. 40 obese women were selected and randomly divided into 4 groups of combined e training, CLA supplementation, combined training and CLA supplementation and control .combined training program 3 days a week with an intensity of 55-75% of a 1RM, and 55 to 75 maximum heart rate. The supplement and exercise group received daily 4 g CLA supplements and control group received 4 g of placebo daily. After 8 weeks and 48 hours after the last training session, all variables were re-measured. In order to statistical analysis, t-test was used and covariance analysis was used to check the difference between groups. the significance level of the tests was considered to be P≤0.05. All computing was performed by using SPSS software version 22. After eight weeks of combined training and conjugated linoleic acid supplemented were decreased the serum levels of resistin, glucose, insulin and insulin resistance and body compositin. (P≤0.05). Fasting glucose, insulin and insulin resistance, and body composition indices were significant between groups(P≤0.05). 8 weeks combination training and CLA supplementation with decrease serum level of resistin, Fasting Glucose ,insulin resistance and Body composition and control weight decreased The risk of type 2 diabetes, atherosclerosis, and the risk of cardiovascular disease in obese women.

Helicobacter Pylori (H.pylori) is a flagellated, spiral- shaped, microaerophilic gram negative bacterium that colonizes stomach mucosa and causes upper gastrointestinal diseases, such as gastric inflammation, chronic gastric ulcers and gastric Carcinoma. Chronic inflammation plays an important role in the pathogenesis of autoimmune diseases, allergies and cancer. Inflammatory cytokines, such as IL-19 play an important role in the pathogenesis of diseases caused by H. pylori. Since Helicobacter infection is very important and effects many subjects for a long period, and since it has the potential of converting into cancer, we attempted to examine the role of IL-19 cytokine in induction and continuation of this disease. Materialand methods: In this case-control study, blood samples of 100 patient's and 100 healthy individuals was examined for gene polymorphisms of IL-19 at rs1028181-513T/C position in patients with H. pylori infection. Genotype of this polymorphism was determined by RFLP PCR method. Statistical data analysis of genomic and serum studies of patients was carried out by the X 2 (Chi square) and logestic regression. Investigation of relationship between T and C alleles at rs1028181-513T/C position indicated that there is a significant correlation between allele C and H. pylori infection (p = 0.04 OR = 0/66 (CI; 0/45-0.96)). It seems that there is a direct association between Helicobacter Pylori infection, and C allele at rs1028181-513T/C position.

Helicobacter pylori infection is highly rampant and approximately half of the world's population is infected with this bacterium. Urea breath test (UBT) is the most important non-invasive method for diagnosing Helicobacter pylori. This study aims to evaluate frequency of H. pylori infection in Salmas County using Urea Breath Test (UBT). In this cross-sectional survey, 80 people were studied with symptoms of nausea and vomiting, chronic gastritis and history of positive Ig G response who had filled out a checklist regarding to UBT. In this study, Spss21 software were used for Chi-square test and T-test to analyze statistical data. 37 people out of 80 were infected with H. pylori (46%) and 43 had no infection (54%). Groups aged between 31-40 years old and 60-51 years old were included in high risk groups for infection and risk of gastric cancer. The study revealed, there was a significant relationship between smoking and women's education level with the incidence of infection in patients. The results of this study showed that there is a indirect correlation between H. pylori infection and one’s education level. By increasing the level of knowledge and health awareness associated with the factors involved in the development of the disease, the prevalence of H. pylori infection can be reduce to a desirable level.

Today, we are dealing with the waves including, the waves emitted from wireless devices connecting to the Internet, which can have a different negative impact on human health. The aim of the present study was investigated the effect of electromagnetic waves emitted from the tablet connected to internet on the movement and number of sperm in adult male rat. 40 male rats were divided into two control and treatment groups with electromagnetic radiation emitted from a tablet connected to the Internet, respectively. Sperm count and sperm motility in both groups were analyzed by T-test. The results showed that the electromagnetic waves emitted from the tablet connected to the Internet caused a significant decrease in the total number of sperms and had a significant effect on sperm motility. In the treatment group with electromagnetic waves from tablet, the number of healthy and damaged sperm had significantly decrease and increase respectively compared to the control group, but there was no significant difference between semi motile sperms in the two experimental groups. It can be concluded that the electromagnetic waves emitted from the internet-connected tablet lead to reduced count and motility of sperm.

Diabetic foot ulcers, which are complications of diabetes mellitus, can be accompanied by an infection in the wound, which can increase the risk of amputation or even death. E coli bacteria, which have been reported to be present in diabetic foot ulcers, can lead to worsening of the condition of the wound. The aim of this study was to detect β-lactamase genes in Escherichia coli isolated from diabetic foot ulcer by Multiplex PCR in Jahrom city. Determination of antibacterial sensitivity of Escherichia coli isolated using disc diffusion method. The detection of strains producing ESBL enzymes was performed using Double disk synergy test. In order to detect carbapenem resistant organisms in the TSF, the concentration of 0.5 McFarland was used. To isolate and identify MBL-producing strains, the Etest MBL (AB BioDisk, Solna, Sweden) tapes was used according to the manufacturer's instructions. In a multiplex PCR assay, using specific primers, the presence of genes encoding beta-lactamase enzymes including blaTEM, blaSHV and blaOXA-1 was studied.

Aim and sedentary lifestyle and high-calorie foods lead to obesity, hence the aim of the study was to investigate the effects of different doses of exercise and caloric restriction on insulin receptor substrate-1 in obese male rats. Methods and materials: 48, eight-week-old male Wistar rats (190 ± 16 g) fed high-fat palate for seventeen weeks. After obesity induction, the obesity group was randomly assigned to one of the six groups: EXE, CR, EXCR1, EXCR2, and EXCR3 as experimental groups and OC as the control group. Then 10% of food intake was reduced. Exercise intensity was adjusted about 70-75% vo2max, 28 m/min for 48 to 55 minute. Negative energy balance was applied through caloric restriction (CR) (6d/w), exercise training (EXE) (6d/w), and in three combinations: EXCR1 (2d/w exercise + 4d/w CR), EXCR2 (3d/w exercise + 3d/w CR) and EXCR3 (4d/w exercise + 2d/w CR) for two month. One way ANOVA and Tukey post-hoc was applied for Data analysis by SPSS version 16 (p<0.05). IRS-1 mRNA expression was significantly higher in CR in comparison to OC (p=0.024). Also EXCR2 IRS-1significantly increased comparing to OC (p=0.033).weight in CR, EXCR1, 2,3 were significantly reduced compared to OC or HC. 10% of negative energy balance in forms of caloric restriction per se or in conjunction with exercise can provoke mRNA expression of insulin substrate receptor-1 that may lead to insulin sensitivity improvement.

Backgraound: The process of programmed cell or apoptosis is a naturally occurring process in the body. This process ocurrs during different phases of morphogenesis in embryonic and adulthood periods. However, it must be carefully controlled to avoid some potential diseases. The abnormal changes that affect the normal behavior of cellular components can activate the immune system. Theses changes may trigger the process of apoptosis and unusual changes in cellular components, which is the cause of many diseases. It is worth noting that many chemical compounds, including curcumin, have the ability to regulate and control apoptosis. Also, there is evidence that aerobic activity, especially with moderate intensity, plays a protective role in the process of apoptosis. This study provides a detailed overview of the research done on the impact of curcumin and aerobic activity with moderate intensity and different protocols on apoptosis. In this study, we reviewed more than 100 studies on herbal medicine and physical exercise that have been published from 1995 to 2017. The present review article indicates that long-term, moderate-intensity aerobic activity is effective in reducing apoptosis. Also, the effect of curcumin on the reduction of apoptosis is dependent on the consumption dose, and up to 200 mg / kg can reduce apoptosis. This review can be used as a basis for the combined use of traditional medicine and physical activity to optimize the process of apoptosis.