فصلنامه محیط زیست جانوری
سال هفتم شماره 4 (زمستان 1394)

An important tool in the study of the physiological state of an organism is the blood. The present study aimed to determine and record the variants between plasma and serum biochemical parameters in Great sturgeon (Huso huso), Persian sturgeon (Acipenser persicus) and Caspian roach (Rutilus rutilus caspicus) was performed. To achieve this goal, sturgeon and Caspian roach weighted average 3-5 and 2-3 g, were provided from Shahid Marjani Proliferation and Culture Center for Sturgeon Fish and Sijwal (Golestan province) and were kept in Research Center of Agriculture and natural Resources, Gorgan University. All of the experimental factors during the growing period (water temperature: 23 C˚, pH 7/5 and DO: 7/9; Fish were fed 2 times a day with commercial food) were the same for tanks. To examine the blood biochemical factors of serum and plasma (aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), albumin, total protein and glucose), blood samples were collected and blood biochemical parameters was performed according to standard methods. There were no significant differences in the values obtained from the study of alanine aminotransferase (ALT), creatine phosphokinase (CPK), and albumin of all three species (Great sturgeon, Persian sturgeon and Caspian roach) (P≥ 0.05). Plasma albumin levels were lower than serum plasma in all three species, whereas plasma ALT levels despite the lack of significant difference (P≥ 0.05) in all three species were more than serum ALT. Serum and plasma glucose levels showed significant differences (P≤ 0.05) which in all samples, blood plasma glucose levels were higher than serum glucose levels. The AST factors in all three species obtained values from plasma were greater than those obtained from serum. Serum total protein was greater than total plasma protein obtained in all three species and also the differences in roach was significantly higher than plasma total protein (P≤0.05). Since the observed differences between some factors of serum and plasma in the present study, seems due to the greater similarity of blood plasma to natural blood status, the use of plasma is preferred to study in these three species.