فصلنامه گیاه و زیست فناوری ایران
سال پنجم شماره 4 (زمستان 1389)

Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4M NaCl). H. salinarum contains ions in the growth conditions of the bacteria. This paper reports the effects a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. The homogeneity of the protein was considerably affected by variant of various parameters of the culture، including light، aeration، and stirring، on the structure of bacteriorhodopsin. The protein obtained under condition was analyzed by electrophoresis. In the last few years Bacteriorhodopsin (bR) became probably one of the most studied proteins due to its high stability and a variety of properties which are suitable for electronics، optics and other applications. The main purpose of research is isolation of bacteriorhodopsin in the form of nanostructure of PM with a high purity degree. And degree of magnesium ion effect is surveyed in the proton pump. Halobacterium salinarum is cultured in the stress non-aerobic and radiation conditions. Then the bacteriorhodopsin protein is analyzed in the isolating PM nanostructure from by electrophoresis. For surveying the effect of magnesium ion in the activity extent of proton pump، the pH of the environment was controlled by pHmeters. Purity degree of isolated bacteriorhodopsin protein in PM nanostructure form is 2. 1. The physiological density of magnesium ion influenced on the activity extent of proton pump and in its present. The activity of proton pump will be increased. The applied methodology in research، is a proper method for isolation of bacteriorhodopsin in large scale and there in no need to complex equipments. But the purity degree of resulted PM is not higher than 2. 1.

Electricity is one of the advanced and technology keys. In recent decades using of generating devices of electromagnetic fields has led to placed humans exposed to electromagnetic fields. Vitamin E is one of the soluble antioxidant in fat that has an important role in lipoproteins، the biological membrane، nervous system، reproductive system and antioxidant chain the is placed against oxidative damage. In this research the antioxidant effects of vitamin E on the repressing of induced chromosomal damages by extremly low-frequency electromagnetic field on bone marrow erythrocytes of adult male Balb/C mouse has been studied. In this experimental study، 31 adult male Balb/C mice were divided into 3 groups control، shamexposed and experimental groups. control group were kept in natural conditions، Mice in sham- exposed group were exposed to low electromagnetic field intensity 50Hz for 4 days، each day 4 hours and the mice in experimental group were injected intraperitoneally، vitamin E with dose of 200 Mg / Kg for 8 consecutive days، and the fifth day، these mice were exposed to electromagnetic fields intensity 50 Hz for 4 days، each day 4 hours. after completion of treatment، all mice were dissected and micronucleus test was evaluated in poly chromatic erythrocytes of bone marrow. Quantitative data obtained using of Spss software and ANOAA test at the level of pResult