فهرست مطالب

زیست شناسی کاربردی - سال بیست و هفتم شماره 2 (پاییز و زمستان 1393)

نشریه زیست شناسی کاربردی
سال بیست و هفتم شماره 2 (پاییز و زمستان 1393)

  • تاریخ انتشار: 1393/09/12
  • تعداد عناوین: 7
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  • Pages 5-18
    Quantitative and qualitative capabilities of biomass production of Artemia in polyethylene tankers with 1.2 m 3 diluted Inche salt lake water set at salinity of 60 ppt, and average initial stocking density of 2700 N/Lit. were evaluated during a culture period of 21 days in a batch culture system. A mixed diet including small amount of green algae Dunaliella, baker’s yeast and rice bran (as main food source) were used as food source. At the end of the experiment following results were obtained: an average total length of 8.02 mm, final survival of 25.5 percent, and an average biomass production of 2276 gr/m3. A feed conversion ratio of 0.41 was recorded for rice bran and yeast. Artemia reached a final weight of 3.45 mg at the end of the experiment. In the view point of nutritional value, produced biomass showed a protein content of 37.5 percent, while its fatty acid profile was rich in fatty acids with 18 carbons in their chain.
    Keywords: Artemia biomass, Green algae, Growth, survival, Nutritional value
  • Pages 19-30
    Number of methods developed for remediation of heavy metals from aqueous effluent, but generally they require high investment, operation and maintenance costs. Recently many studies have been done for exploration cost effective ways for removal of heavy metals. One of the effective technologies to remove heavy metals is to use bio-absorbents which are economical, easy to access, and conformed to environmental standards. The aim of this research was to study the biosorption level of nickel using Gracilaria sp. In this study, the batch removal of nickel (II) ions from aqueous solution under different experimental conditions using of Gracilaria was investigated. The nickel (II) uptake was dependent on initial pH, with pH 5 being the optimum value. The equilibrium data were fitted using Langmuir isotherm model. The maximum nickel sorption was attained within the first 15 min and the sorption equilibrium was reached after 120 min.
    Keywords: Biosorption, Gracilaria, Nickel, Isotherm, Langmuir
  • Pages 31-48
    In this study, the protective effects of an herbal drug known as STW 5 (Iberogast®) -in dose dependent manner- on oxidative lung damages in rats induced by experimental sepsis was assessed. For this purpose, the rats were divided into six groups: Sham-operated (SOP); CLP; CLP + STW 5 (2.5, 5 &10 mg/kg) and CLP + Indomethacin (positive control). The drugs were injected i.p immediately after sepsis induction and the lung tissues were examined histophatologically 24 hour after CLP. Based on the qualitative and quantitative histological data, it was found that induction of sepsis was associated with extensive infiltration and sequestration of PMN and also high histologic index of the severity of lung injury. In compare to Indomethacin, the treatment of rats with STW5 did not improve these parameters. The data shows that STW 5 cannot modulate pathological parameters leading to lung damages in CLP rat model.
    Keywords: STW 5, acute inflammation, CLP model, Histopathology, Lung
  • Pages 49-72
    Loranthaceae comprises three hemiparasitic genera with five species in Iran. The genera of this family occur mainly in tropical and subtropical areas worldwide. These genera family are distributed in two geographical regions of Alborz and the central parts of Iran. The taxa existing in Iran which are included in this analysis are: the genus Loranthus Jacq., which includes two species: L. europaeus, which lives on Quercus, and L. grewinkii is found on Pyrus and Amygdalus; the genus Viscum L. with two species: V. album and V. laxum attacking different trees; and Arceuthobium oxycedri which is found on Juniperus spp. Jin jungles of Semnan. The total DNA was extracted from fresh or herbarium specimens with a modified CTAB method. The regions of nrDNA ITS and cptrnL-F were amplified and sequenced. In this study, 27 taxa (24 taxa for cptrnL-F, 23 taxa for nrDNA ITS, 20 taxa for combined sequence data) were studied as ingroups and 3 taxa as outgroups. To reconstruct the phylogenetic relationships in Loranthaceae and to compare the affinities of this family with the other members of Santalales, the molecular data were analyzed. The analyses were carried out using Maximum Parsimony, Bayesian method and Maximum Likelihood method. Our results indicated that Loranthaceae is monophyletic. The strict consensus tree from Maximum Parsimony analysis of the concatenated two gene dataset showed two monophyletic clades with 100% bootstrap support for two families Loranthaceae and Viscaceae.
    Keywords: Molecular phylogeny, Loranthaceae, Viscaceae, Parasitic plants, ITS, trnL, F
  • Pages 73-84
    In aquaculture egg incubation is a most critical time as fish are vulnerable to environmental factors, so characterization of antioxidant enzymes development in this stage of life is a great scope of recently researches. Stocking density induced stress that affects energy intake, growth rate, enzymatic activity and oxygen consumption in fish. In this study the development of some antioxidant enzyme (GPX, CAT and SOD) in serum of rainbow trout (Oncorhynchus mykiss) that kept in different stocking density was investigated. Lipid peroxidation and serum concentration of MDA evaluated as well. Eggs were held in tanks supplied with flow-through freshwater at 10.8̊C in triplicate. Samples were taken at day1 (fertilization), 3 (Cleavage), 8(organogenesis), 16 (eyed egg), 31 (hatch) and 48 (active feeding). A significant increase in GPX and SOD activity was seen from fertilization till eggs were eyed. (P<0.001).Activity of these enzymes decreased to active feeding (P<0.001), meanwhile the activity was higher than fertilization. Our results demonstrated that catalase activity increases from fertilization to organogenesis and then toward active feeding significantly decreased in compare to fertilization (P<0.001). Overall trend of MDA concentration showed significant increases from fertilization toward active feeding in all groups (P<0.001).
    Keywords: Catalase, Superoxide dismutase, Glutathione peroxidases, Malondialdehyde, Oncorhynchus mykiss
  • Pages 85-106
    Salicylic acid (SA) is a naturally produced compound and has been implicated to play important roles in tolerance of plants against diverse biotic and abiotic stresses. Experiments were conducted to study the regulatory role of SA on antioxidant enzyme induction in two salt sensitive (Y3000) and relative salt tolerant (H420) cultivar of canola. The experiment was conducted in Hajiabad country, Qom. The seeds were cultivated as a completely randomized block. SA treatment carried out with 0, 0.5 and 1μM of SA solution in the early morning when the plants fourth leaf completely expanded. The samples were taken at up to120 (vegetative phase) days after germination for analysis. The results revealed that salicylic acid increased peroxidase, polyphnol oxidase and superoxide dismutase activities in Y300 cv. By contrast antioxidant enzymes activities decreased in H420 cv. PAGE gel electrophoresis analysis of antioxidant enzymes revealed that salicylic acid increased peroxidase activity and intensity in Y 300 cv. Poly phenol oxidase activity was unchanged and Cu,/ Zn-SOD was the most abundance isozyme. The activity of this izozyme increased in treated plants. Fe- SOD was observed in Y 300 cv. and Mn-SOD was observed in H420 cv. Therefore ROS involve in different signaling pathway in different compartments of the cell in salt sensitive and relative salt tolerant cultivars. As a result SA increased salt tolerance in salt sensitive cultivar by increasing antioxidant enzyme activity.
    Keywords: Salycilic acid, Canola, Antioxidant enzymes, salt stress
  • Pages 107-117
    Pasargadae set of ancient structures remaining from the Achaemenid impire are located in Fars province, Iran. Tomb of Cyrus the Great, which is made of limestone placed in the southern part of this area. Like any other stone, the building has been influenced by physico-chemical and biological factors. Biodeterioration of materials is an irreversible process which materials and equipment under the influence of the micro and macroorganisms are degraded and causes massive economic losses. Almost all microorganisms are in the destructive biofilm form on the external surfaces of the monuments and phototrophic microorganisms preparing suitable condition for the growth of other organisms. The effects of these organisms are particularly important in the context of biodeterioration. BG-11, MKM, BBM medium was used for isolation of phototrophs. Stereomicroscope and scanning electron microscope (SEM) was done to observe the biological structures and the pattern of deteriorating alteration on the surface of the substratum. To identify the phototrophic populations, cultivation method was used. During this study, several species of microorganisms, including algae and cyanobacteria in biofilm structure, were isolated. Most of these species were sampled from the inside of tomb that displays more suitable conditions for phototrophic growth. Stereomicroscopic observation and SEM images revealed that microorganisms were better located within the cavities of the stone, where the intensity of light is optimum for phototroph growth. In this study, the most frequent type of biodeteriorant algae belong to Trebouxia sp., Chlorococcum sp. and Trentepohlia sp. For cyanobacteria, the genus of Chroococcus sp., Oscillatoria sp., Microcystis sp., Aphanothece sp., andLyngbya sp. were dominant cyanobacteria. The first step in eliminating orcontrolling the growth of deteriorating microorganisms is their isolation andidentification. Thus, the present study was carried out to isolate and identifyinvolved microorganisms. Further investigation should be done to find the bestmethod to clean the surfaces of the stone.
    Keywords: Algae, Cyanobacteria, Phototrophic microorganism, Biodeterioration Pasargadae