فهرست مطالب
Journal of Food Quality and Hazards Control
Volume:4 Issue: 4, Dec 2017
- تاریخ انتشار: 1396/10/09
- تعداد عناوین: 8
-
-
Pages 93-98BackgroundThere is growing demand to improve physicochemical, microbiological, and sensory properties of fresh foods using natural herbal antimicrobial and antioxidant compounds. The aim of the present study was to investigate antioxidant and antibacterial properties of some native edible plants of Kermanshah, Western Iran.MethodsThe methanolic extracts of leaves of Falcaria vulgaris, Allium rotundum, Tragopogon graminifolius, and Mentha longifolia plants were prepared. The antibacterial effects of these four plant extracts were determined on Salmonella typhimurium, Bacillus subtilis, B. cereus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7 using micro-broth dilution and agar disk diffusion assays. Also, the 2,2-diphenyl-1-picrylhydrazyl hydrate assay was used for determination of antioxidant properties of the plant extracts. The analysis was performed using SPSS 16.0 (Chicago, IL, USA) software package.ResultsThe most antibacterial effectiveness was significantly (pT. graminifolius>A. rotundum>F. vulgaris. Moreover, Gram-negative bacteria were more resistant to the presence of methanolic plant extracts than Gram-positive bacteria. The highest antioxidant activity (based on IC50) was significantly (pConclusionThe studied edible plants had antimicrobial and antioxidant activities that recommend as potential preservatives in food products. However, methanolic extract of M. longifolia had the best antibacterial and antioxidant properties in vitro.Keywords: Anti-Bacterial Agents, Antioxidants, Plants
-
Pages 99-102BackgroundIt is known that Aflatoxin M1 (AFM1) contamination in milk can have carcinogenic, teratogenic, and mutagenic activities especially in liver for both children and adults. The main aim of this survey was to investigate the incidence of AFM1 in human and cow milk in Kashan, Iran.MethodsA total of 42 breast milk samples were collected from a central hospital in Kashan city during 3-month period from October to December 2012. In the same period of times, 48 cow raw milk samples were collected randomly from Kashan milk collection centers. Determination of AFM1 in samples was carried out by competitive enzyme linked immunosorbent assay. Data were analyzed by SPSS software version 16.0.ResultsAll human and cow milk samples were contaminated with AFM1. Concentrations of AFM1 in all human breast milk samples were less than the maximum tolerance limit (25 ng/L) accepted by European :union: (EU). In 20.83% raw milk samples, the level of AFM1 was greater than the maximum tolerance limit (50 ng/L) according to EU. Mean AFM1 concentration in cow milk samples was significantly (pConclusionComparing to the previous Iranian studies, although the present status of AFM1 in human and cow milk samples from Kashan city of Iran is not at high risk, but this finding dose not ignore the vital importance of exposure risk to this toxin in the consumers especially in children. So, protective diet oriented approaches must be considered by the local authorities. Also, the people must be educated by the government on public health risk of AFs.Keywords: Aflatoxin M1, Milk, Enzyme-Linked Immunosorbent Assay, Iran
-
Pages 103-108BackgroundLiterature review shows that there are not sufficient data about polyphenolic compounds of peel of Iranian pomegranate. So, this work was mainlyundertaken to determine phenolic compounds and antioxidant activity of dried peel of Iranian pomegranate.MethodsPomegranate fruits were obtained from mature fruits grown in Saveh, Iran and the Pomegranate Peel (PP) were dried with three different methods. Powders of PP were extracted with four different solvents, using a soxhlet apparatus. The compounds of PP extracts were analyzed by High Performance Liquid Chromatography (HPLC). Then, yield percentage and Radical Scavenging Activity (RSA) were determined. Statistical analysis was performed using the SAS 9.1 software.ResultsDifferent ranges of tannic acids, testosterone and α-estradiol, estriol, estrone, cyanidin 3-glucoside, cyanidin3,5-diglucoside, pelargonidin 3-glucoside, pelargonidin 3,5diglucoside, and delphinidin 3-glucoside were identified. Both the highest yield percentage (18.820±0.661) as well as the highest RSA percentage (63.862±0.376) were obtained from the ethanol showing significant (p0.05) relationship with yield of extraction and also antioxidant activity of the PP extracts.ConclusionHPLC analysis identified some various phenolic compounds in Iranian PP extract showing considerable antioxidant activities. Although drying method showed no relation with yield of extraction and also antioxidant activity of the PP extracts, but type of solvent was effective on yield of extraction and type of extracted compounds of PP.Keywords: Phenol, Antioxidants, Punicaceae
-
Molecular Identification of Listeria monocytogenes in Raw Hamburgers from Kerman, South-East of IranPages 109-112BackgroundListeria monocytogenes is a Gram-positive and facultative anaerobic food-borne bacterium which is capable of intra and extra cellular growth. L. monocytogenes usually can exist on different surfaces and instruments at production and processing sites of food products with animal origin. In Iran, the consumption of burger has increased recently, but its safety is still of great concern. Despite few reports from some areas of Iran, there is limited information about burger contamination in Kerman province. Therefore, this research was set to molecular identification of L. monocytogenes in hamburgers distributed in Kerman, Iran.MethodsA total of 100 raw hamburgers were collected from 20 fast food/sandwich shops in Kerman city, Iran during summer 2014. The hamburgers stored in ice box and transported to the food hygiene laboratory. The samples were microbiologically analyzed for the presence of L. monocytogenes. The isolated bacteria were confirmed by molecular assay.ResultsThree out of 100 (3%) hamburger samples were biochemically diagnosed as Listeria contamination; however molecular identification assay confirmed that two of them were L. monocytogenes.ConclusionAlthough the prevalence rate of L. monocytogenes was not high in hamburger samples of Kerman, the risk of human listeriosis must not be ignored or underestimated. Further surveys are required in future for risk assessment of this pathogenic bacterium in other food products distributed in the country.Keywords: Listeria monocytogenes, Meat Products, Polymerase Chain Reaction, Iran
-
Pages 113-118BackgroundIran could be considered as one of the most important producers of honey all over the world. This investigation was conducted to evaluate some chemical quality characteristics of honey produced in Iran.MethodsTotally, natural (n=80) and commercial (n=20) honey samples were randomly collected from North-West and South-West regions of Iran. Hydroxymethylfurfural (HMF) levels, phenolic contents, antiradical activity, and antioxidative potency of the samples were analyzed. Data were statistically analyzed using SPSS version 16.ResultsThe HMF level in 4 out of 80 (5%) natural honey samples and 14 out of 20 (70%) of commercial honey samples was higher than the recommended safety limit set by the Iran national standard. The average phenolic content was significantly (pConclusionIn conclusion, HMF levels, phenolic contents, antiradical activity, and antioxidative potency of Iranian natural honeys were remarkably more acceptable and suitable than commercial honeys produced in the country.Keywords: Antioxidants, 5-hydroxymethylfurfural, Honey