فهرست مطالب

Journal of Applied Biotechnology Reports
Volume:3 Issue: 3, summer 2016

  • تاریخ انتشار: 1395/06/28
  • تعداد عناوین: 7
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  • Jafar Khezri, Fathollah Ahmadpour, Samira Talebi Pages 437-440
    Bacterial utilize a multitude of methods to secretion of specific substrate across phospholipid membranes. These bacterial strategies can play many roles in promoting bacterial process, from enhancing pathogenesis to bacterial response to environmental condition. The secretion in bacterial species transfer and release performance substance such as proteins, enzymes and metabolites to environment. The process of secretion plays an important role in the performance and compatibility with environment. Many bacteria use dedicated protein secretion systems to secrete from the cytosol into environment. In general, bacterial protein secretion systems classified to different classes, there are at least six specific secretory systems in Gram-negative bacteria. In this study, we review type I and II secretory systems of Gram-negative bacteria.
    Keywords: Secretory System, Gram, Negative Bacteria, Bacteria Membrane
  • Alireza Zebarjadi, Hossein Rostami Ahmadvandi, Danial Kahrizi, Kianoosh Cheghamirza Pages 441-445
    Syrian Rue or Harmal (Peganum harmala L.), belonged to the family of Peganaceae, grows in semi-arid climates such as the Middle East and North Africa. Traditionally, this plant, especially the seeds, has been recognized for its several medicinal uses. In this stydy, genetic diversity between 21 Harmal accessions, collected from different regions of Iran were evaluated by Inter Simple Sequence Repeat (ISSR) marker using 14 spesific primers. All primers successfully amplified polymorphism regions, as among 115 regions, 68 polymorphic regions (59.13%) were amplified. Accordingly, highest and lowest similarities among ecotypes were measured as 94% and 55%, respectively. The results based on cluster analysis also categorized all accessions into three groups that did not completely match to their geographic pattern place of collection. Result of principle coordinate analysis of samples also showed distribution pattern similar to cluster analysis.
    Keywords: Peganum harmala, Genetic Diversity, ISSR, Cluster Analysis
  • Sahar Shakuri, Ali Mohammad Latifi, Morteza Mirzaei, Samaneh Khodi Pages 447-451
    Bacteriorhodopsin operates as a light/proton transfer pump which converts the light energy into a proton gradient. Energy stored in the proton gradient can be used in a variety of ways. Bacteriorhodopsin producing sources are some Halobacterium species such as Halobacterium sodomense and Halobacterium salinarum which continue to grow in harsh and salt-saturated conditions. Two types of red pigment (IRLS.1) and orange pigment (IRLS.2) producing strains were isolated in order to isolate bacteriorhodopsin producing strains from Aran-Bidgol Lake. Spectroscopy reviews and the results of SDS Page of membrane proteins of two isolated strains and Iranian native Halobacterium salinarum showed that there is bacteriorhodopsin protein in the sample. Spectroscopic studies showed that Halobacterium salinarum produces the maximum amount, then IRLS 1 and finallyIRLS.2 with the lowest amount of bacteriorhodopsin. The results of biochemical and molecular identification based onthe16srRNA of both mentioned strains indicated their highest similarity with Natrinema sp. XA3-1 and Archaeon RC34, respectively. In this study, the presence of bacteriorhodopsin protein in Iranian native strains was examined for the first time and was isolated purely from Halobacterium salinarum membrane by gel filtration chromatography that given the widespread use of bacteriorhodopsin protein, it will be so effective.
    Keywords: Bacteriorhodopsin, Rhodopsin, Purple Membrane, Halobacterium, Retinal, FPLC
  • Khadige Abadian, Zohre Keshavarz, Faraz Mojab, Neda Bromand Pages 453-456
    Primary dysmenorrhea is a prevalent issue and it can reduce the quality of life in many females worldwide. Based on the properties of Teucrium polium as a herbal medicine with analgesic and inflammatory properties, this triple-blind, randomized, placebo controlled trial was performed. The study aimed to evaluate the effects of Teucrium polium on the severity of primary dysmenorrhea in students. In this study, single students were categorized in two groups who received Teucrium Polium (n=35) or placebo (n=35). For the first 3 days of menstruation, subjects were asked to take some capsules containing powder Teucrium polium (250 mg) four times daily for two consecutive menstrual cycles. Pain severity was examined using a visual analog scale. According to the results, there was no significant difference in the pain severity at the baseline between two groups. Pain severity was significantly decreased in both groups after the intervention; however, larger pain reduction was significantly seen in the group which had received Teucrium Polium (p
    Keywords: Placebo, Dysmenorrhea, Teucrium Polium, Herbal Medicin
  • Ali Daryabeigi Zand, Hassan Hoveidi Pages 457-463
    Phytoremediation has gained increasing attention in recent years because of its ability to clean up and redevelop brownfield sites. Petroleum hydrocarbon-contaminated sites are among most prevalent industrial sites in Iran as one of the top oil producers in the world, which are in great need of remediation to prevent associated environmental and human health risks. A greenhouse study was conducted to identify the tolerance of sunflower (Helianthus annus L.) and its growth parameters under various scenarios. Additionally, remediation potential of sunflower in spiked soil with low to moderately gasoline contaminated soil was evaluated. Results indicated that sunflower is a tolerant plant species in low to moderately gasoline-contaminated soil with remarkable biomass establishment in contaminated soil and acceptable phytoremediation potential. Sunflower was found to tolerate gasoline contamination up to the maximum applied concentration of 5000 mg/kg. However, sunflower biomass and height slightly decreased in presence of gasoline in soil which were not significant in most cases (P>0.05). Significant reduction of gasoline under the influence of sunflower was obtained in all vegetated treatments when compared to non-vegetated treatments (P0.05). Results of the present research indicated that gasoline content of soil cannot be considered as a controlling factor affecting phytoremediation potential of sunflower when soil is polluted with low to moderate levels of gasoline. Soil remediation with sunflower can be considered as a promising approach to manage moderately gasoline-contaminated sites.
    Keywords: Gasoline, Phytoremediation, Plant Growth, Spiked Soil, Sunflower
  • Fahimeh Mohamadi Suni, Mohammad Farkhari, Henghameh Taheri, Payam Pour Mohammadi, Mohammad Barzegari Pages 465-472
    Since crosses of lines with suitable genetic distance produce excellent hybrids, the identification of genetic diversity between Zea mays L. inbred lines in hybrid maize breeding programs has a high priority. A total of 27 inbred lines plus B73 and Mo17 (Sc7 parents) were evaluated by 15 inter-simple sequence repeat (ISSR) primers. Inbreds were selected randomly from inbred lines from the Agriculture & Natural Resources Research Center of Khuzestan. A total of 107 polymorphic bands (85.5%) were detected out of 127 bands with an average PIC of 80%, which shows an acceptable variation in the genetic material of the center. Dendrograms were produced based on simple matching similarity coefficients and furthest neighbor (complete linkage) clustering algorithm. In the dendrogram created, genotypes were categorized into three groups. At least two heterotic groups can be identified among maize germplasm from this center. The results of the current investigation, despite the lack of pedigree information for the lines studied, could shed light on the management and utilization of these lines to develop new hybrid varieties.
    Keywords: Maize inbred lines, Genetic diversity, Heterotic groups, ISSR
  • Morteza Hamedi Behnaq, Ali Karami, Ali Choopani Pages 473-476
    Abrin known as ribosome inactivating protein’s (RIP) it is a high cytotoxic plant protein. High lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. Because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. As a result, remaining of specific toxin gene in these extracts take the advantage of using PCR assay to identify abrin gene, that refers to exist of its toxin. We use new rapid molecular method for abrin gene detection by PCR. We designed specific primers for detection of abrin toxins gene by PCR. DNA was extracted by cetyl trimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method from Rosary pie samples and PCR protocol was performed using primers for toxin gene amplification. Then we analyzed assay’s sensitivity by serial dilution method. The results of this study revealed that designed and selected primers sequence for toxin’s gene is specific. The desired Product size was obtained and Sequencing of PCR products was performed that show up to 90% similarity with known sequence for each molecule. According to these results, the developed rapid molecular method for detection of abrin toxin gene is sensitive, and low-cost to detect this toxins gene in cases of suspected to bioterrorism event.
    Keywords: Abrin, PCR, RIP