Diversity and phylogenetic studies of H. vulgare and its subspecies according ACC1 and ACC2 gene

Barley belongs to the Poaceae which is the largest monocotyledon family. Plastid single-copy gene acetyl- coAcarboxylase (ACCase) is the first step in the biosynthesis of fatty acids and therefore it is used to study the phylogenetic relationships, evolutionary and systematc of grasses. Single and low copy genes are less likely subject to concerted evolution, thus making themselves ideal tools for studying the origin and evolution of polyploid taxa. In this study, phylogenetic relationship of the species of H. vulgare with its subspecies; H. spontaneum, H. distichon, H. tworow, H. hexastichon, were investigated using gene-specific primers acetyl- CoAcarboxylase, ACC1 (plastid form) and ACC2 (cytosolic form). There was high adaptation between homogeneity test and disparity index with phylogenetic trees. The homogeneity of substitution patterns between H. vulgare with its subspecies sequences has not been seen. In conclutsion the common tree between two genes revealed that ACC1 and ACC2 have the same evolution pathway.