Comparative study on the effect of obtained cell wall and cytoplasmic fraction from Saccharomyces cerevisiae and Saccharomyces boulardii on K562 cell line

' The goal of this study was to evaluate in vitro anti turmeric properties of extracted cell wall and cytoplasmic fraction from Saccharomyces cerevisiae and S. boulardii as probiotic and comparative their effect on chronic myeloid leukemia (K562) cell line. S. cerevisiae and S. boulardii were cultured in a medium containing yeast extract and disrupted with sonicator and centrifuged for separation the cell wall from cytoplasmic fraction. Then different concentrations of cell wall and cytoplasmic fraction were prepared and their anti tumeric properties assayed with MTT technique by using Doxorubicin as positive control. Our result showed both cell wall and cytoplasmic fraction that obtained from S. cerevisiae and S. boulardii have anti turmeric effect on K562. Statistical analysis indicated that Yeast cell wall could inhibit the growth of K562 than cytoplasmic fraction at level of p<0.05. Also, we found that, prepared compounds from S. boulardii have high anti turmeric properties than S. cerevisiae, but there was not seen any differences (p<0.05). Based on obtained results we conclude that the extraction of cell wall from both microorganisms has suitable application for inhibiting chronic myeloid leukemia, but these anti tumeric properties would warrant further study on the clinical application of yeast cell wall.