Cytotoxity Evaluation of Docetaxel Nanoparticles Against HepG2 Cell Lines

Background and Human hepatocellular carcinoma (HCC) is one of the most common causes of death in the world. Docetaxel (DTX) is one of the most active chemotherapeutic agents fortreating of many kinds of cancer. The purpose of this study is to investigate the properties of docetaxel cytotoxicity of nanoparticles against HepG2 cell lines. In this study, emulsion polymerization method using thiolated chitosan and cerium ammonium nitrate as initiator is prepared for oral consumption HepG2 cells were seeded in 96-well plates at the density of 10,000 viable cells per well and incubated 24 h to allow cell attachment. The cells were incubated with docetaxel loaded thiolatedchitosan nanoparticle and dug free suspension at 0.01, 0.1, 1, 10, and 50 µg/ml for 24, 48 and 72 h, respectively. The prepared nanoparticles showed a suitable size for drug delivery with posetive zeta potential. Inhibitory concentrations (IC50) was obtained for nanoparticles and free drug during incubation times of 24, 48 and 72 h respectively. The results showed higher cytotoxity effect of nanoparticles in comparison with free drug against HepG2 cell lines in all mentioned incubation times. These results suggested that thiolated-chitosan nanoparticles could be a potentially useful delivery system for docetaxel as an anticancer agent for thetreatment of liver cancer.