In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting

The in-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) was evaluated. Trypsin is known to cause belly bursting in common kilka. The assessments showed that in a range of 4-70°C, trypsin from pyloric caeca and intestine of common kilka had the maximum activity and the satiability at 60 and 55°C, respectively. The pH assessments indicated that maximum activity and stability for trypsin were at 8. 5 at pH range of 4-11. The effects of metal ions on trypsin activity revealed that CaCl2, MgCl2 and MnCl2 increased trypsin activity while CuCl2، ZnCl2 and Al2 (SO4) 3 decreased its activity. The effect of inhibitors on trypsin activity also showed that SBTI and TLCK (specific inhibitors for trypsin) significantly inhibited trypsin activity. This study suggests that belly bursting in common kilka can be prevented by trypsin inactivation through application of low temperature (4°C), acidic pH, metals of CuCl2, ZnCl2 and Al2 (SO4) 3 and inhibitors of SBTI and TLCK.