Production of canola protein concentrate: III- hydrolyzing selected processed products of canola meal (Brasscia napus) using different inclusion levels of phytase and washing with ammoniated methanol

Using canola meal in spite of suitable protein compound is limited due to existunce of some antinutritional compounds such as fiber, phytate, glucosinolate and tannin. This study aimed to evaluate the effect of phytase and washing with ammoniated methanol on the chemical composition (crude protein, phytate, glucosinolate, tannin, acid detergent fiber and neutral detergent fiber) of three processed products of canola meal with hemicellulase (0.10 and 0.15% CM) and pectinase (0.15% CM) at three doses (4000, 5000 and 6000 IU/Kg, n=3) of phytase in a factorial design. With an increase in phytase concentration, phytate content decreased and crude protein content increased. Washing with ammoniated methanol decreased the glucosinolate content of all processed products of canola meal with carbohydrases (4.93 vs 1.98 μmole/g dry matter) and tannin content (1.53 vs 1.14%) but it increased contents of crude protein (41.87 vs 42.79%), phytate (2.06 vs 3.02%), acid detergent fiber (24.50 vs 26.84%) and neutral detergent fiber (27.35 vs 29.11%). Processed product with pectinase 0.15% CM, phytase and ammoniated methanol had the lowest content of glucosinolate (3.26 μmole/g dry matter) compared to those of others. Finally, the relative priority of treatments were determined based on the weighted average using principal component analysis as pectinase (0.15% CM) and 5000 and 6000 IU phytase/Kg and then pectinase (0.15% CM) with 4000 IU phytase/Kg.