P27 AND/OR CYCLIN-D1 BIOMARKERS AND CLINICOPATHOLOGICAL PARAMETERS OF BREAST CARCINOMA

Breast cancer is the most common malignant tumor in women. In our country (Iran) based on the study of the “Institute of cancer”، breast cancer accounts for 10. 8% of all newly diagnosed cancers. Because of different clinical course of breast cancer in sufferers، identifying the factors that directly or indirectly predict the ultimate outcome of patients is useful for clinical management and determining the prognosis. In recent years much attention has been paid to the use of biomarkers in predicting treatment response and prognosis. All the mastectomy specimens with the diagnosis of “breast invasive ductal carcinoma” in in Imam Khomeini Hospital were selected between 2004 to 2008. Five slides of each paraffin block were prepared، one of which stained for H&E، and the rests stained for Cyclin-D1، P27، and ER/PR according to Dakocytomation system. Slides stained by Haematoxylin and Eosin were assessed to determine tumor histologic grade based on the Nottingham modification of the Bloom-Richardson grading system. Regarding the expression of estrogen receptors، 14 cases (36. 8%) were ER positive and 24 patients (63. 2%) were ER negative، and for expression of progesterone receptors، 14 cases expressed PR (36. 8%) but 24 patients (63. 2%) were PR negative. 18 patients were positive for P27 and 9 patients expressed Cyclin-D1 positively (23. 7%). According to the obtained results، no significant correlation were not seen between P27 and/or Cyclin-D1 expression and clinicopathological parameters of breast carcinoma such as patient’s age، number of the involved axillary lymph nodes، histologic type or grade of the tumor، tumor size and steroid receptors expression. Finally، we suggest further investigation on relationship between these biomarkers and other prognostic factors in a larger group.