Design and Construction of plastid Transformation vector contain BADH gene

Transgenic plastids offer unique advantages in plant biotechnology، including high-level foreign protein expression and posing lower environmental ricks. As regard to sequencing of lettuce plastid genome، many Homologous segments can be used for vector designing to production recombinant proteins in this plant. We have described the development of a plastid transformation system for lettuce، (Lactuca sativa L. TN-96-39-B). The transforming DNA carries a betaine aldehyde -resistance gene BADH cassette (lettuce rrn promoter-BADH- lettuce psbA terminator) under control of lettuce chloroplast regulatory expression elements، flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. This system will open up new possibilities for the efficient production of plasma proteins، enzymes، growth factors، recombinant edible vaccines and antibodies in lettuce.