The Survey of Potential of B1 Aflatoxin Isolation by Lactic Acid Bacteria

Mycotoxins are poisons which produce by soil mould funguses and are abundant in the environment and food stuff. The most important group of these toxins is Aflatoxins which are produced by some mold species from the Aspergillus genus. The most dangerous and strongest is B1 Aflatoxin with different toxic impressions including human being liver cancer. Researchers have revealed that a group of none pathogenic bacteria named as Lactic Acid Bacteria are able to connection the B1 Aflatoxin and other Aflatoxins and Mycotoxins discharge them from environment and food stuff. The purpose of this research to obtain Lactic Acid Bacteria that their ability to help in connection and isolation B1 Aflatoxin is used them as food supplements or probiotic products for prevent of absorption B1 Aflatoxin in human and animal body. In the present research the bacteria are isolated from five groups of different sources. For survey their bacteria ability for isolation of B1 Aflatoxin used ELISA method and used for identification the result strains 16S rRNA sequencing method by apply universal primers. Among the strains isolated two strain Lactobacillus pentosus and Lactobacillus berevis ability of B1 Aflatoxin absorption and isolation that respectively are abile to absorb and discharge 17.4 and 34.7 percent of B1 Aflatoxin from the experiment solution. Isolated Lactobacillus pentosus from human feces and Lactobacillus berevis from local milk sample. For survey significant of the result Used SPSS 16 software.