Serological and Molecular Detection of Tomato spotted wilt virus (TSWV) in Khorasan Razavi Province

In order to investigate the presence of Tomato spotted wilt virus (TSWV) in tomato fields of Khorasan Razavi province (Mashhad، Neishabour، Torbat-e-Hydarieh، Kashmar، Ghochan، Fariman، Takht-e-Golgeh and Roshtkhar)، totally 636 samples were collected from tomato fields in summer of 2008 and 2009. The plant that showed symptoms of chlorotic، necrotic and ring spot were collected and transferred to the laboratory in cold condition، then tested by TAS-ELISA. Sap from infected plants inoculated to 3 cultivars of indicator plant e. g Nicotiana rustica، N. clevelandii and N. tabacum var Samsun. To confirme symptomology، inoculated indicator plants tested by TAS-ELISA. For molecular detection، RNA extracted by use of RNX TM (plus) kit. In RT-PCR، specific primer، amplified a fragment of 276 bp. Using RT-PCR and mechanical transmission tests، the presence of TSWV has been proven in tomato field of the province. In inoculating indicator plants only Nicotiana tabacum var Samsun (leaf deforming and mottling) showed specific virus symptoms.