Taxonomical relationship of maize dwarf mosaic virus, isolate of Golestan province, with cereal potyviruses based on 3 region of the genome

Maize dwarf mosaic virus (Maize dwarf mosaic virus) is one of the most important and widespread maize potyviruses in the world. In order to determine the taxonomic position of the isolates of the virus in Golestan province among cereal potyviruses and other strains of MDMV and its relationship with strains of other countries, corn and johnsongrass plants with mosaic symptom and dwarfing were collected from fields of Golestan province. Samples were verified using an antiserum against MDMV by indirect ELISA. The viral RNA was isolated from an infected sample and the 3΄-region of the genome of virus was amplified using potyviral degenerate and specific primers by RT-PCR.The obtained consensus sequence for CP-UTR region was 1126 nucleotides. Comparison was made between these isolates and a number of other cereal potyviruses available in GenBank and phylogenetic tree was constructed by neighbor-joining majority- rule method. In phylogenetic analysis 46 sequences were grouped into 6 viruses: SCMV, PenMV, IJMV, SrMV, ZeMV and MDMV. In this tree, isolate of Golestan were grouped with isolate of Bulgaria. Based on this analysis the Iranian isolate of MDMV has been probably originated from Europe or more probable from Mediterranean basin