Effect of metabolites produced by Lactobacillus rhamnosus GG (probiotic bacteria) on the growth of human colon cancer cell line CacoII

In this study the inhibitory effect of metabolites produced by Lactobacillus rhamnosus GG (probiotic bacteria) on the growth of human colon cancer cell line CacoII was examined. After accuracy examination of bacterial genus and species MRS(DE Man, Rogosa & Sharpe) broth culture from L. rhamnosus GG was prepared and two supernatant from MRS broth culture which was incubated for 24,48 and 72 hours were provided.One of these supernatant with normal pH(pH=4) and the other one with using NaoH 1N was reached to pH 7.CacoII cells were seeded on 96-well microplates at 8×103 cells/well and obtained supernatants at concentrations of 100,200 and 300 µl/mL were inoculated to CacoII cell culture. In average, the metabolites of L. rhamnosus GG at concentrations of 100, 200 and 300 µl/mL inhibited the growth of CacoII cells by 60.8%,71.5% and 82.1% respectively.