Preparation and structural assessement of PHB/nHA composite scaffold used in bone tissue engineering

In This study, hydroxyapatite nanoparticles (nHA) was synthesized via sol-gel method and then poly 3-hydroxybutyrate\Hydroxyapatite Nanocomposite scaffolds (PHB/nHA) prepared by solvent casting and particulate leaching Technique. The scaffolds were prepared from 0.4-0.8 gr PHB in 10mm choloroform. nHA weight percent was 0-10 and the ratio of PHB:NaCl was 30:70, 20:80, 10:90. synthesized nHA powder and nanocomposite scaffolds were characterized by XRD, FTIR, SEM, WDXA and TEM. nHA particle size was 30-40 nm and 35 nm according to TEM image and debbye sherrer equation rerpectively. XRD parren confimed HA formation. FTIR analysis shows that there is a chemical bond between polymer and nHA particles that causes strength of scaffold. SEM results shows that morphology, size and distribution of pores is uniform and pore size is 200-250 mm. uniformity of pores is better in 4%wt of PHB \Choloroform solution and decreasing of salt amount, causes increasing of closed pores on the surface.Theoric porosity of prepared scaffolds was obtained 57-84 for 70-80 %w NaCl that is closed to measures obtained by diffusion method. uniform distribution of nHA in scaffolds were investigated by wavelength dispersive spectroscopy (WDXA). This study showed that (PHB/nHA) composite scaffold is a good candidate for bone tissue engineering.