Bacteriophage as a new candidate for the prevention and removal of biofilms

Pseudomonas aeruginosa is a ubiquitous organism which has emerged as a major threat in the hospital environment. Overuse of antibiotics has also significantly increased the emergence of antimicrobial multiresistant bacteria. P. aeruginosa has an innate ability to adhere to surfaces and form virulent biofilms. Bacteriophage might represent one attractive solution to this problem. In this study، P. aeruginosaphage was utilized to Biofilm prevention and removal. After isolation of phages and serial dilution microplate method of biofilm formation was examined. Furthermore، the effects on biofilm bacteria isolated phages were determined on the Pseudomonas putida، Acinetobacter baumanii and E. coli. P. aeruginosa biofilm had OD: 1. 688 in 492n. m. Pure phage، 10-2 and 10-3 diluted phage decreased OD to 1. 587، 1. 341 and 1. 461،respectively. Isolated phage dramatically decline OD of Biofilm of all strains. Phages have various affinity to attach to hosts، thereby it is supposed to phages compete for their receptors. Therefore it is supposed phages have most efficiency in optimum concentration to remove biofilm or growth inhibition. Also، funding prove phages have effect on various bacteria and they can use to remove different strains.