Combined Application of Microbial Cellulose and Papaver macrostomum Extract on Bedsore Microorganisms

Bedsore is one of the major problems in all the societies as patients are confined to bed. Due to antibiotic resistant strains being a significant obstacle for cure, many plants and herbs are being used by researchers as medicinal compounds. The investigation of synergistic effect of cellulose biopolymer and Papaver macrostomum extract on bedsores bacterial community. Acetobacter xylinum PTTC 1734 was cultured in Schramm-Hestrin (SH) medium and incubated at 30°C for 24-48 hours. NaOH treatment and absolute ethanol were used to extract cellulose biopolymer and plant antimicrobial substance, respectively. The Biopolymer structure was scanned by a Scanning electron microscope (SEM). Antimicrobial activities, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of these extracts were all determined separately. The effective concentration of each extract's alone, combined, and synergistic effects were evaluated. Biopolymer absorption efficiency was assayed as the absorbent bed. Pesudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus were the dominate bacteria isolated from bedsore samples. Antimicrobial effects of cellulose, P. macrostomum extract, and the combination of both were determined on the isolated bacteria as 1, 10, and 15 mm respectively. 100-1000μl/mL of flower ethanol extract concentrations of P. macrostomum indicated the maximum effect on mixed bedsore's bacteria rather than leaf and mixed extraction. Concentrations 500 1000μl/mL decreased the bacterial bedsore's growth and completely inhibited it. 3.5g/L of cellulose biopolymer was obtained from A. xylinum broth culture medium. Scanning electron microscopy analysis confirmed the branched structure of this polymer. Cellulose absorption efficiency was evaluated to be 14.5ml/g in this investigation. Because of high-absorbance of bio-cellulose, combined plant extraction with this biopolymer caused a decrease in the growth of bedsore microorganisms with the minimum extract concentration, 100μl. Combination of bio-cellulose and P. macrostomum flower ethanol extract can be used for patients who suffer from bedsore lesions in concentration 0.1% of MBCs. Furthermore, clinical studies are needed to confirm the efficiency of in vivo application.