Evaluation of bene hull extraction antioxidant activity by superheated water method

In the present research, the power of antioxidant activity in Bene Hull subspecies (Mutica) extract was discussed using subcritical water technique, at 110,155 and 200°C temperatures with 45 minutes of constant time and the ratio of 1: 30 for Bene hull to water. The dependent variables of this study were measured based on Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) and total amount of phenolic compounds (Mg of Gallic Acid/ 100 G of material). The study revealed that at 200°C the Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) were at the maximum; meanwhile total Phenolic content was highest at 155°C. Under this circumstance, the amount of polyphenolic contents, reduction power according to EC50 and DPPH free radical scavenging activity according to EC50 were predicted to be 2096.41 mg Gallic acid / 100 g Bene hull, 0.213 mg /ml and 0.685 mg/ ml, respectively. The results proved that subcritical water extraction method was suitable to obtain biological materials out of Bene hull. Intense extraction yield, abundant solvent, cheap and non-environmental issues are its benefits.