Expression Clustering of Proteins of Alzheimeric and Normal Rat Hippocampus Treated with Lavandula Angustifolia

One of the shortcuts to disco-ver drug targets is the utilizing of new kn-owledge of basic medical sciences such as genomics، proteomics، metabolomics، bio-informatics and biostatistics that are apply-ing holistic approaches to study biological and medical subjects. In this study pro-teomics، bioinformatics and biostatistics techniques were applied to identify prob-able therapeutic proteins targets for the treatment of Alzheimer''s disease. Hippoc-ampus proteomes of normal and Alzhe-imeric rats treated with aqueous extract of lavender were evaluated with protein expr-ession clustering method.

Proteins of hippoc-ampus samples from normal and Alzheim-eric rats that treated with aqueous extract of lavender (CE and AE) and without the extract (C and A) were extracted. Proteins were separated by using of two-dimensional gel electrophoresis and the gels were stai-ned by silver staining methods. Bioinfo-rmatics and biostatistics analysis of the separated were accomplished by bioinfo-rmatics software.

Bioinformatics and statistical analysis of the stained gels obtained 990 proteins in the four groups of rats. Prote-omic comparison of A and AE groups reve-aled that the expression of 49 proteins was inhibited، while the expression of 26 new proteins in the AE group was observed that may be due to the present of lavender extract. Proteomic comparison between gro-ups C and CE showed the expression of the 80 new proteins and inhibition of 104 pro-teins. Discussion &

After the remov-al of common proteins between the two groups، those proteins that were affected by the extract assigned as drug targets for the treatment of Alzheimer. Changes at the molecular level were revealed with multi-variate statistical analyzes such as principal component analysis or correlation analysis and the clustering of proteins showed that the expression changes have been occurred in three main clusters. Finally، it could be concluded that lavender extract caused significant expression changes in the pro-teome and possibly activated specific biological processes in the rats’ hippoc-ampus that associated with enhancement of learning and memory in normal and Alzh-eimeric rats.