Comparison of two IVF and Piezo-ICSI experimental methods from standpoint of embryo development in outbred NMRI mouse

In order to do in vitro fertilization (IVF), 435 oocytes were collected from 20 super ovulated NMRI female mice, in M2 media with 4% BSA. 85% (n=370) of collected oocytes were in MII phase. Epididymal sperms were added to 332 oocytes in T6 media drops with 4% BSA and incubated. Fertilization rate was 79.8% (n=265) out of which 27.5% (n=73) grew to morula and 20.8% (n=55) achieved blastocyst stages.For Piezo- intracytoplasmic sperm injection (Piezo-ICSI), 20 super ovulated female mice were sacrificed and 355 oocytes were collected in M2 media with 4% BSA. 83% (n=295) of collected oocytes were in MII phase. Epididymal sperms were piezo-injected to cytoplasm of 213 MII oocytes and incubated. 62.4% (n=133) of injected oocytes were fertilized and 14% (n=30) degenerated. 39% (n=52) of fertilized cells grew to morula and 12.8% (n=17) to blastocyst stages embryos at laboratory conditions. In this study the fertilization rate of gametes, growth and survival rate of laboratory embryos were evaluated in vitro. Results show higher fertilization rate and the laboratory embryos with desirable quality and quantity were obtained by the use of IVF technique from conventional-outbreed laboratory mouse, that is economical and easy as compare to Piezo-ICSI. However, outbred laboratory NMRI mouse that can be easily bred and kept in conventional conditions is recommended for use in oocyte recovery and embryo production in assisted reproductive technology.