Effect of Hsp90 adjuvant on immunogenicity of HbsAg in BalbC mice

Heat shock proteins، (Hsps) are used as adjuvants in vaccines; these proteins induce the inflammatory cytokines and the maturation of monocyte MQ to dendritic cells. This study was done to determine the effect of Hsp90 on the immunogenicity of HbSAg in BalbC mice. In this experimental study we injected Hsp90 β subunit together with HbsAg to 30 balbC mice. After one and two weeks the humoral and cellular systems of these animals and their controls were studied by ELISA. There were significant rise in serum total IgG and IgG2a in the experimental group as compared to controls. Hsp90 also caused an initial increase and subsequently a significant fall in the level of IL-4. Hsp90 β subunit can be considered as a proper adjuvant in the formulation of hepatitis B vaccine. In future we should test its protective effect on BalbC mice.