Molecular analysis of gene coding Psoralen synthase as a key enzyme in medicinal plant Psoralea corylifolia

Psoralen is one of the important secondary metabolites in Psoralea corylifolia. The important aspect of medicinal value of psoralen can be indicated to anticancer activity against leukemia, lung and breast cancers. In this research due to the pharmaceutical importance of psoralen, pathway of psoralen synthesis was studied. For the first time we isolate and characterized the gene coding psoralen key enzyme from the leaves of the Psoralea corylifolia by the different molecular techniques. Result of molecular analysis revealed that the length of this gene was 1237 bp. Also, the sequence analysis of nucleotides by using available bioinformatics data in NCBI found that psoralen synthase gene has 93% similarity with this gene in Ammi magus.