Effect of resistance exercise on protein content and mRNA expression of NT 4/5 in rat slow and fast muscles

Trophic factor family plays a key role for neuromuscular system healthy. This study was carried out to determine the effect of one session of resistance exercise on protein content and mRNA expression of NT4/5 in rat slow and fast muscles. In this experimental study, sixteen adult male rats randomly were allocated into resistance exercise (T) and control groups. The resistance training protocol consisted of climbing a 1-meter–long ladder, with a weight attached to a tail sleeve. Quantitative Real time RT-PCR for NT-4/5 expression and ELISA Kit for protein assay were used. Resistance training significantly decreased mRNA expression and increased protein of NT4/5 in soleus muscle (P<0.05). Significant alteration was not detected in flexor hallucis longus muscle. One session of resistance training can alter protein and mRNA of NT-4/5 in skeletal muscle and this alteration was dependent on muscle type.