The effect of HLADRB1 subtypes, on clinical diseases activity index in rheumatoid arthritis patients referred to rheumatology clinic of Shahrekord University of Medical Sciences

Rheumatoid Arthritis (RA) is a common inflammatory joint disease with substantial morbidity, specially, if remained untreated. Genetics, environmental and immunologic factors have important roles in pathogenesis of disease. The aim of this study was to investigate the effect of HLADRB1, its subtypes on clinical disease activity index (CDAI) and to diagnose the frequency of HLADRB1 alleles in RA patients. In this descriptive analytic study, 64 patients with RA were selected based on ACR criteria by convenience sampling. All patients were studied to assess primary CDAI. Patients, DNA was extracted by using phenol chloroform method. Then it was assessed HLADRB1 based on (SSP- PCR) method by innotrain kits made in Germany. After preparing results of lab data, patients was examined for the second stage (6 month later) and completed questionnaires for each patient, and analyzed hypothesis of study. The common HLADRB1 in patients was HLADRB1 (04) in frequency 31.3%. In patients with HLDRB1 (04), (01),(15), CDAI decreased after 6 month(p<0.05), but it was not observed in others patients significant(p>0.05). This study showed the multi-factorial character of RA patients may be present. It was found out that the patients with HLADRB1 (04), (01), (15) have good response to routine treatments, against the references that seems HLADRB1(04) have no decrease in secondary CDAI. Perhaps environmental and genetic factors affect very important in etiology. Supplementary surveys could complete and promote these results.