Teratogenic and toxicity effects of ~
Carboxin on morphology and liver cells of chicken embryo

Inadequacy of foods for human societies has led to an irregular increment of pesticides usage in agriculture. In this study، the teratogenic effects of Carboxin، a broad spectrum fungicide in agriculture، was evaluated on chicken embryo. Carboxin was dissolved in acetone and injected into yolk sac in concentrations range of 20- 400 µg/egg. The compound could cause death and a number of abnormalities in the embryos. Skeletal staining showed the caudal vertebrates were not calcified and the long bones were significantly shortened and distorted. In concentrations of 200 and 400 µg/egg، the main external abnormality was clubfoot. Treatment of the liver cell culture with carboxin showed a significant antiproliferative activity. In the concentrations of 5، 10، 25، 50 and 100 µg/ml، the cell viabilities were 80، 76، 65، 60 and 52%، respectively، showing the IC50 of 102 µg/ml for the compound.