Identification of the fauna of plant parasitic nematodes associated with grape in Dena, Iran

In order to identify the plant parasitic nematodes associated with grape in Dena, Kohgiluyeh and Boyer Ahmad Province, Iran, about 50 soil and root samples were collected from different parts of the region during 2011-2012. The nematodes were extracted from soil samples using tray and centrifugal flotation techniques. The extracted nematodes were fixed and transferred to glycerin and the permanent slides were prepared. The fixed nematodes were examined by light microscope equipped with drawing tube and identified considering the morphologic and morphometric characters with the help of scientific keys and references. In this study, 8 species from 7 genera were identified as Aphelenchus avenae, Boleodorus thylactus, Helicotylenchus macronatus, Mesocriconema antipolitanum, M. xenoplax, Pratylenchus andinus, Psilenchus hilarulus and Seinura prospera of whichP. andinus is a new report from Iran and B. thylactus, H. macronatus, M. antipolitanum, M. xenoplax and S. prospera are new reports from Kohgiluyeh and Boyer-Ahmad Province.