Genetic Diversity of Chilo supperssalis (Lepidoptera: Pyralidae) Populations Based on RAPD Marker in Guilan Province, Iran

To investigate the genetic diversity of Chilo suppressalis populations in Guilan province، a number of 37 of samples (4-6th larva stages) were collected from 17 different regions of the province. The collected samples were parted into three population groups coming from west، center and east parts of Guilan. Extracted DNAs were polymerized through 12 RAPD markers. RAPD-PCR products were electrophoresed on a 1. 5% agarose gel and photographed through GelDoc. Following bands scoring، analysis of data revealed that the 12 primers produced a total of 112 polymorphic bands. Results indicated that C. suppressalis population differs from the other two populations. Center and east Guilan populations demonstrated more similarities to each other. This could be due to an occurrence of gene-flow between the two populations causing the similarities. The population coming from the center of Guilan demonstrated a highest level of intrapopulation genetic variation due to a wider activity area and as well due to a higher number of the studied samples. These results finally indicated that population’s active in the province lack homogeny and are comprised of two different biotypes. In total، the results could be considered as an explanation for morphological and biochemical differences found among the active biotypes within the studied areas.