Restorative Effect of Iranian Probiotic Bacteria Lactobacillus casei on Healing gastric Stomach Ulcers Caused by Acetic Acid in Male Wistar Rats

Gastric ulcer is a common disease caused by male nutrition and drug side effects. The treatment with chemical drugs is along with Physiological problems. Problems such as self -immunity has brought many efforts for finding new treatments for Gastric ulcer. Probiotic bacteria are the dominant digestive organisms used in the treatment of digestive diseases. One of the most important groups of probiotic bacteria is lactic acid، normally used as the ferment of dairy products The aim of this study is to investigate the restorative effect of probiotic bacteria isolated from native dairy products on healing gastric (stomach) ulcer of male wistar rats. In this study، the mice were randomly divided into three groups including the experimental، control 1 and control 2 groups. After tolerating Twenty-four hours of hunger، mice received surgery and Gastric (stomach) ulcer was created through injection of 12 / 0 ml of acetic acid (60%v/ v). One day after the surgery، the experimental group received 10 × 1010 cfu / day bacterial solution in one ml of sterilized milk، and control groups were respectively treated by one ml of sterilized milk and 2 ml of water through gavage until the time of dissecting. the mice were killed at 1، 4، 7، 10 and 14 days after building the stomach ulcer. The statistical analysis was performed by SPSS software and test results as Mean ± SEM were reported. The strains of bacteria Lactobacillus casei have significantly decreased the dimensions (sizes) of gastric (stomach) ulcer of the experimentalgroup than the control group 1 and 2 (p < 0 / 001) and have increased gastric ulcer healing. Thehistological studies on the fourth day showed a significant increase of neutrophils، macrophages and fibroblasts in the experimental group than control groups and on the 7th، 10thand 14thday showed significant reduction of neutrophils and macrophages in the experimental group in comparison with control groups. Fibroblasts were significantly increased in the experimental group on 7thday and decreased in 10thand 14thday in comparison with control groups. The probiotic bacteria Lactobacillus casei have a significant restorative effect on gastric ulcer induced by acetic acid.