Discrimination of Entamoeba Spp. in children with dysentery
Author(s):
Abstract:
Aim
The present study was performed in order to differentiate E. histolytica and E. dispar in children from Gorgan city, using a PCR method.Background
Differential detection of two morphologically indistinguishable protozoan parasites Entamoeba histolytica and E. dispar has a great clinical and epidemiological importance because of potential invasive pathogenic E. histolytica and non-invasive parasite E. dispar.Patients and Methods
One hundred and five dysentery samples were collected from children hospitalized in Taleghani hospital in Gorgan city. The fecal specimens were examined by light microscopy (10X then 40X) to distinguish Entamoeba complex. A single round PCR amplifying partial small-subunit rRNA gene was performed on positive microscopy samples to differentiate E. histolytica/ E. dispar and E. moshkovskii from each other.Results
Twenty-five specimens (23.8%) were positive for Enramoeba complex in direct microscopic examination. PCR using positive controls indicated E. histolytica and E. dispar in two (2/25, 8%) and three (3/25, 12%) samples, respectively.Conclusion
There is a warrant to performing molecular diagnosis for stool examination at least in hospitalized children in order to prevent incorrect reports from laboratories and consequently mistreating by physicians.Keywords:
Entamoeba histolytica , Entamoeba dispar , children , dysentery , PCR , Gorgan , Iran
Language:
English
Published:
Gastroenterology and Hepatology From Bed to Bench Journal, Volume:7 Issue: 3, Summer 2014
Page:
164
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