Emdogain effect on gingival fi broblast adhesion in bioabsorbable and non-resorbable barrier membranes: An in vitro study

Tissue engineering represents very exciting advances in regenerative medicine; however, periodontal literature only contains few reports. Emdogain (EMD) consists of functional molecules that have shown many advantages in regenerative treatments. This study investigated EMD effect on gingival fi broblast adhesion to different membranes. Two dense polytetrafl uoroethylene membranes (GBR-200, TXT 200), Alloderm and a collagenous membrane (RTM Collagen) were used in this experimental study. Each membrane was cut into four pieces and placed at the bottom of a well in a 48-well plate. 10 μg/mL of EMD was added to two wells of each group.Two wells were left EMD free. Gingival fi broblasts were seeded to all the wells. Cell adhesion was evaluated by means of a Field Emission Scanning Electron Microscope after 24 hours incubation. Data was analyzed by independent t-test, one-way and two-way ANOVA and post hoc LSD test. P < 0.05 in independent t-test analysis and P < 0.001 in one-way ANOVA, two-way ANOVA and post hoc LSD analysis was considered statistically signifi cant. Alloderm had the highest cell adhesion capacity in EMD+ group and the difference was statistically signifi cant (P < 0.001). In EMD- group, cell adhesion to TXT-200 and Alloderm was signifi cantly higher than GBR-200 and collagenous membrane (P < 0.001). This study showed that EMD may decrease the cell adhesion effi cacy of GBR 200, TXT-200 and collagenous membrane but it can promote this effi cacy in Alloderm. It also showed the composition of biomaterials, their surface textures and internal structures can play an important role in their cell adhesion effi cacy.