Biocontrol of Causative Agent of Citrus Canker Disease Using Antimicrobial Substances Produced by Aspergillus awamori K-03

Bacterial phytopathogens have a great impact on yield loss. In between, the causative agent of citrus canker Xanthomonas citri subsp. citri (Xcc), has influenced citri culture worldwide. Antimicrobial efficacy of Aspergillus awamori K-03 secrotome was tested against 26 strains of Xanthomonas citri ssp. citri using disc diffusion method. The secretome managed to control other Gram positive and negative bacterial pathogens including Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, Salmonella typhi, Staphylococcus aureus and Erwinia amylovor. The secretome was heat stable and remained active in wide ranges of pH (4-9).Discussion and Our result demonstrates that the A. awamori K-03 secretome has antimicrobial activity and can be used as a biocontrol agent.