Effect of Medium, Sugar and Plant Growth Regulators on Micropropagation of Saint Julien A (Prunus domestica spp. Insititia) Rootstock

Saint Julien A (Prunus domestica spp. Insititia) is known as one of the important semi-dwarfing rootstocks with good compatibility with the majority of stone fruits (especially with plum)، which no enough research carried out of effective factors on its micropropagation. In this project the effect of 5 types medium (MS، WPM، TK، GNH and mGNH)، the interaction effects of PGRs (BAP at concentration of 0. 25، 0. 5، and 1. 0 mg/l and IBA at concentration of 0. 0، 0. 05، 0. 1، and 0. 2 mg/l])، and also the effect of carbohydrate source (Sucrose and Glucose) each at concentration of 0. 0، 10، 20 g/l were studied on mentioned rootstock shoot promotion separately. Besides، for root induction two different methods application of IBA and NAA (gradually pre-application and pulsing) was independently conducted. Results indicated that the highest healthy shootlets N0. (2. 125 per each explants) were observed on MS medium supplemented with BAP and IBA at concentrations of 0. 5 and 0. 1 mg/l، respectively، which had significant effect (α=0. 05) in comparison with other treatments. Inspite، the result implicated clearly the significant interaction effects (α=0. 01) of sucrose and glucose on both number and length of shootlet، in which the highest shootlet N0. (2. 66) with desired growth quality was obtained when MS medium supplemented with both carbohydrates each at 20 g/l concentration، but the longest shoots (8. 5 mm) were observed on MS containing 30 g/l sucrose which had significant difference (α=0. 05) in comparison with other treatments The obtained results from root induction step significantly supported the reasonable efficiency of pulsing method (with 77. 28% rooting and 1. 8 root on each shoot) to other conventional method، as well as the superiority of NAA to IBA. The rooted plantlets with 60% success were acclimated in pots containing mixture of perlite and pitmass (3:1) and were transferred into greenhouse.