Assessment of Genetic Relationships Among 36 Brassica Genotypes using ISSR Molecular Markers

Study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. Since the hybrid canola have more yield than inbred lines, determine the genetic diversity of this have particular importance. In the present study, genetic diversity among 36 genotype of Brassica sp. were determined using inter simple sequence repeat (ISSR) markers. Thirteen selected primers produced 267 discernible bands, with 257 (96.38%) being polymorphic, indicating considerable genetic diversity among genotypes. Average PIC and MI of all primers were 0.34 and 6.48, respectively. The results showed that average of marker index including Nei’s gene diversity, Shannon’s information index, the effective number of alleles were 0.34, 0.51, 1.58 respectively in genotypes of Brassica. Based on an un-weighted pair-group method using arithmetic average (UPGMA) clustering algorithm, three distinct groups were established. Genetic diversity shown by the marker ISSR, indicates that these markers can be used to identify differences between species and within species, In studies of phylogenetic. In order to evaluate the relationships and genetic structure of genotype canola using morphological characteristics is proposed.