Detection of rotavirus genome by new silver staining method
Author(s):
Abstract:
Background And Objective
Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years. This study was designed to evaluate the detection of rotavirus genome by new silver staining method using polyacrylamide gel electrophoresis (PAGE). Method
In this descriptive study, the samples were collected from infected MA-104 cell culture and the RNA electrophoresis was performed in 10% polyacrylamide slab gels after RNA extraction. Results
According to polyacrylamide gel electrophoresis and sensitive staining analysis, rotavirus RNA segments were divided into 4 groups and single-nucleotides differences were clearly detected rapidly. Conclusion
New silver staining method using polyacrylamide gel electrophoresis has the capacity to detect the rotavirus electeropherotype within a few minutes even in small DNA/RNA pieces up to 7 picograms.Keywords:
Language:
Persian
Published:
Journal of Gorgan University of Medical Sciences, Volume:16 Issue: 4, 2014
Pages:
126 to 130
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