Evaluation of genetic diversity and classification of advanced sunflower lines using ISSR markers

Sunflower is one of the most important oilseed crops. Evaluation of genetic diversity and grouping of genotypes and lines are computed as important agents for plant breeding programms. In this study, the genetic diversity and classification of some advanced inbred lines of sunflower developed during different breeding programs were studied using ISSR markers. Among 21 primers, 15 ISSR primers were selected for their reproducibility. A total of 70 bands were produced through ISSR primers which 27 and 43 bands out of them were monomorphic and polymorphic, respectively. Results revealed that primer UBC 807 (0.4) had the highest polymorphism information content value and primer UBC 804 (0.15) had the lowest value. Using Dice similarity coefficient the lowest amount of genetic similarity 0.65) was observed between breeding lines SF25 with SF278 and the highest ones (0.93) was observed between lines HA336 and SF315. Cluster analysis using UPGMA algorithm was divided the studied lines into 8 separate groups. Regarding to principal coordinates analysis; each component accounts a small percentage of the total variation which emphasis on good genomic distribution of selected ISSR primers.